Hailu Frehiwet T, Karimpour-Fard Anis, Neltner Bonnie, Stauffer Brian L, Lipshultz Steven, Miyamoto Shelley D, Sucharov Carmen C
Department of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Department of Biomedical informatics, University of Colorado, Aurora, CO 80045, USA.
J Cardiovasc Dev Dis. 2023 Sep 11;10(9):391. doi: 10.3390/jcdd10090391.
microRNAs (miRs) are small non-coding single-stranded RNAs that regulate gene expression. We previously evaluated expression of miRs in the cardiac tissue of children with dilated cardiomyopathy (DCM) using miRNA-seq. However, a comparative analysis of serum and cardiac miRs has not been performed in this population. The current study aimed to evaluate miR levels in the serum of pediatric DCM patients compared to healthy non-failing (NF) donor controls and investigate the association between miR levels in tissue and sera from the same pediatric DCM patients. Defining the relationship between serum and tissue miRs may allow the use of circulating miRs as surrogate markers of cardiac miRs. miR levels were investigated through miR-array in sera [n = 10 NF, n = 12 DCM] and miR-seq in tissue (n = 10 NF, n = 12 DCM). Pathway analysis was investigated using the miR enrichment analysis and annotation tool (miEAA) for the five miRs commonly dysregulated in the sera and tissue of pediatric DCM patients. Functional analysis of miRs commonly dysregulated in the sera and tissue of pediatric DCM patients suggests altered pathways related to cell growth, differentiation and proliferation, inflammation, mitochondrial function, and metabolism. These findings suggest that circulating miRs could reflect altered levels of cardiac tissue miRs.
微小RNA（miRs）是一类调节基因表达的小型非编码单链RNA。我们之前使用miRNA测序技术评估了扩张型心肌病（DCM）患儿心脏组织中miRs的表达情况。然而，尚未对该人群的血清和心脏miRs进行比较分析。本研究旨在评估小儿DCM患者血清中的miR水平，并与健康无心力衰竭（NF）的供体对照进行比较，同时研究同一小儿DCM患者组织和血清中miR水平之间的关联。明确血清和组织miRs之间的关系可能有助于将循环miRs用作心脏miRs的替代标志物。通过miR芯片检测血清中的miR水平（NF组n = 10，DCM组n = 12），并通过miR测序检测组织中的miR水平（NF组n = 10，DCM组n = 12）。使用miR富集分析和注释工具（miEAA）对小儿DCM患者血清和组织中共同失调的5种miRs进行通路分析。对小儿DCM患者血清和组织中共同失调的miRs进行功能分析，结果表明与细胞生长、分化和增殖、炎症、线粒体功能及代谢相关的通路发生了改变。这些发现提示循环miRs可能反映心脏组织miRs水平的变化。
