Qin Yue, Coleman Rhima M
Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
J Funct Biomater. 2023 Sep 11;14(9):468. doi: 10.3390/jfb14090468.
Inducing chondrocyte redifferentiation and promoting cartilaginous matrix accumulation are key challenges in the application of biomaterials in articular cartilage repair. Poly(glycerol-dodecanedioate) (PGD) is a viable candidate for scaffold design in cartilage tissue engineering (CTE). However, the surface properties of PGD are not ideal for cell attachment and growth due to its relative hydrophobicity compared with natural extracellular matrix (ECM). In this study, PGD was coated with various masses of collagen type I or hyaluronic acid, individually or in combination, to generate a cell-material interface with biological cues. The effects of ligand composition and density on the PGD surface properties and shape, metabolic activity, cell phenotype, and ECM production of human articular chondrocytes (hACs) were evaluated. Introducing ECM ligands on PGD significantly improved its hydrophilicity and promoted the chondrocyte's anabolic activity. The morphology and anabolic activity of hACs on PGD were co-modulated by ligand composition and density, suggesting a combinatorial effect of both coating parameters on chondrocyte function during monolayer culture. Hyaluronic acid and its combination with collagen maintained a round cell shape and redifferentiated phenotype. This study demonstrated the complex mechanism of ligand-guided interactions between cell and biomaterial substrate and the potential of PGD as a scaffold material in the field of CTE.
诱导软骨细胞再分化和促进软骨基质积累是生物材料应用于关节软骨修复的关键挑战。聚（甘油-十二烷二酸）（PGD）是软骨组织工程（CTE）中支架设计的可行候选材料。然而，由于与天然细胞外基质（ECM）相比其相对疏水性，PGD的表面性质对于细胞附着和生长并不理想。在本研究中，PGD分别或联合用不同质量的I型胶原蛋白或透明质酸进行包被，以产生具有生物信号的细胞-材料界面。评估了配体组成和密度对PGD表面性质以及人关节软骨细胞（hACs）的形态、代谢活性、细胞表型和ECM产生的影响。在PGD上引入ECM配体显著改善了其亲水性并促进了软骨细胞的合成代谢活性。hACs在PGD上的形态和合成代谢活性受到配体组成和密度的共同调节，表明在单层培养期间这两个包被参数对软骨细胞功能具有联合作用。透明质酸及其与胶原蛋白的组合维持了圆形细胞形态和再分化表型。本研究证明了细胞与生物材料底物之间配体引导相互作用的复杂机制以及PGD作为CTE领域中支架材料的潜力。
