Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
Biotechnology Research Institute, Universiti Malaysia Sabah, 88400, Kota Kinabalu, Sabah, Malaysia.
Probiotics Antimicrob Proteins. 2024 Dec;16(6):2161-2180. doi: 10.1007/s12602-023-10159-2. Epub 2023 Sep 27.
The prevalence of type 2 diabetes mellitus (T2DM) is alarming because it is always linked to the increase in chronic diseases, mortality, and socioeconomic burden. Water kefir has a wide range of functional and probiotic characteristics attributed to the microorganisms present in the kefir grains. The present study aims to evaluate the in vivo anti-diabetic potential of the isolated Lactobacillus paracasei from Malaysian water kefir grains (MWKG) which was reported to have excellent probiotic properties and high antioxidant activities as reported previously. High-fat diet/streptozotocin (HFD/STZ) induction was used to obtain a T2DM model followed by treatment with the isolated L. paracasei from MWKG. The levels of glucose, insulin, and in vivo liver antioxidants were quantified after 14 weeks. Gene expression analysis of the liver was also carried out using microarray analysis, and several genes were selected for validation using quantitative real-time PCR. Insulin tolerance test demonstrated that the L. paracasei isolated from the MWKG alleviated T2DM by improving the area under the curve of the insulin tolerance test whereby low-dose and high-dose concentrations treated groups showed 2424.50 ± 437.02 mmol/L·min and 2017.50 ± 347.09 mmol/L·min, respectively, compared to untreated diabetic mice which was 3884.50 ± 39.36 mmol/L·min. Additionally, treatment with the isolated L. paracasei from MWKG regulated the expression of several genes related to glucose homeostasis and lipid metabolism in diabetic mice. These results suggested that the isolated L. paracasei from MWKG could be a potential dietary supplement for T2DM.
2 型糖尿病（T2DM）的患病率令人震惊，因为它总是与慢性病、死亡率和社会经济负担的增加有关。水克菲尔因其存在于克菲尔谷物中的微生物而具有广泛的功能和益生菌特性。本研究旨在评估从马来西亚水克菲尔粒（MWKG）中分离出的乳杆菌对糖尿病的潜在影响，据先前报道，该乳杆菌具有优异的益生菌特性和高抗氧化活性。高脂饮食/链脲佐菌素（HFD/STZ）诱导用于获得 T2DM 模型，然后用从 MWKG 分离出的乳杆菌进行治疗。14 周后，定量检测葡萄糖、胰岛素和体内肝脏抗氧化剂的水平。还使用微阵列分析对肝脏的基因表达进行了分析，并使用定量实时 PCR 对几个基因进行了验证。胰岛素耐量试验表明，MWKG 分离出的乳杆菌通过改善胰岛素耐量试验的曲线下面积来缓解 T2DM，其中低剂量和高剂量浓度处理组分别显示 2424.50±437.02mmol/L·min 和 2017.50±347.09mmol/L·min，而未治疗的糖尿病小鼠为 3884.50±39.36mmol/L·min。此外，MWKG 分离出的乳杆菌对糖尿病小鼠的一些与葡萄糖稳态和脂质代谢相关的基因的表达进行了调节。这些结果表明，MWKG 分离出的乳杆菌可能是 T2DM 的一种潜在膳食补充剂。
