He Tingyi, Guo Wenrui, Yang Guang, Su Hong, Dou Aolei, Chen Lu, Ma Teng, Su Jie, Liu Moning, Su Budeng, Qi Wangmei, Li Haijun, Mao Wei, Wang Xiumei, Li Xihe, Yang Yanyan, Song Yongli, Cao Guifang
Inner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, China.
Institute of Animal Husbandry, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Huhhot 010031, China.
Vet Sci. 2023 Aug 28;10(9):543. doi: 10.3390/vetsci10090543.
Cell types have been established during organogenesis based on early mouse embryos. However, our understanding of cell types and molecular mechanisms in the early embryo development of Mongolian sheep has been hampered. This study presents the first comprehensive single-cell transcriptomic characterization at E16 in Ujumqin sheep and Hulunbuir short-tailed sheep. Thirteen major cell types were identified at E16 in Ujumqin sheep, and eight major cell types were identified at E16 in Hulunbuir short-tailed sheep. Function enrichment analysis showed that several pathways were significantly enriched in the TGF-beta signaling pathway, the Hippo signaling pathway, the platelet activation pathway, the riboflavin metabolism pathway, the Wnt signaling pathway, regulation of the actin cytoskeleton, and the insulin signaling pathway in the notochord cluster. Glutathione metabolism, glyoxylate, and dicarboxylate metabolism, the citrate cycle, thyroid hormone synthesis, pyruvate metabolism, cysteine and methionine metabolism, thermogenesis, and the VEGF signaling pathway were significantly enriched in the spinal cord cluster. Steroid biosynthesis, riboflavin metabolism, the cell cycle, the Hippo signaling pathway, the Hedgehog signaling pathway, the FoxO signaling pathway, the JAK-STAT signaling pathway, and the Wnt signaling pathway were significantly enriched in the paraxial mesoderm cluster. The notochord cluster, spinal cord cluster, and paraxial mesoderm cluster were found to be highly associated with tail development. Pseudo-time analysis demonstrated that the mesenchyme can translate to the notochord in Ujumqin sheep. Molecular assays revealed that the Hippo signaling pathway was enriched in Ujumqin sheep. This comprehensive single-cell map revealed previously unrecognized signaling pathways that will further our understanding of the mechanism of short-tailed sheep formation.
基于早期小鼠胚胎，细胞类型已在器官发生过程中得以确立。然而，我们对蒙古绵羊早期胚胎发育中的细胞类型和分子机制的理解受到了阻碍。本研究首次对乌珠穆沁羊和呼伦贝尔短尾羊胚胎发育第16天（E16）进行了全面的单细胞转录组特征分析。在乌珠穆沁羊E16阶段鉴定出13种主要细胞类型，在呼伦贝尔短尾羊E16阶段鉴定出8种主要细胞类型。功能富集分析表明，在脊索簇中，TGF-β信号通路、Hippo信号通路、血小板激活通路、核黄素代谢通路、Wnt信号通路、肌动蛋白细胞骨架调控和胰岛素信号通路等多个通路显著富集。在脊髓簇中，谷胱甘肽代谢、乙醛酸和二羧酸代谢、柠檬酸循环、甲状腺激素合成、丙酮酸代谢、半胱氨酸和甲硫氨酸代谢以及产热和VEGF信号通路显著富集。在轴旁中胚层簇中，类固醇生物合成、核黄素代谢、细胞周期、Hippo信号通路、Hedgehog信号通路、FoxO信号通路、JAK-STAT信号通路和Wnt信号通路显著富集。发现脊索簇、脊髓簇和轴旁中胚层簇与尾巴发育高度相关。拟时间分析表明，在乌珠穆沁羊中，间充质可转化为脊索。分子检测显示，Hippo信号通路在乌珠穆沁羊中富集。这一全面的单细胞图谱揭示了以前未被认识的信号通路，将加深我们对短尾羊形成机制的理解。
