School of Civil Engineering, Heilongjiang University, Harbin, 150080, PR China; Engineering Research Center of Rural Water Safety of Heilongjiang Province, Heilongjiang University, Harbin, 150080, PR China.
Heilongjiang Academy of Black Soil Conservation and Utilization, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, PR China.
J Environ Manage. 2023 Dec 1;347:119035. doi: 10.1016/j.jenvman.2023.119035. Epub 2023 Sep 25.
In order to meet the land use requirements of the treated wastewater from high density polyethylene (HDPE) membrane-enclosed anaerobic lagoons, the physical and chemical properties of the wastewater in an HDPE membrane-enclosed anaerobic lagoon were measured. The total nitrogen content (TN) in wastewater treated in membrane-enclosed anaerobic lagoons is 3165 and 1510 mg/L in winter and summer respectively. The wastewater can be used as liquid organic fertilizer, partly replacing chemical fertilizer. The safety dosage of the wastewater was 21.48 t/ha for rice and 9.54 t/ha for corn, respectively. Meanwhile, the wastewater has the characteristics of high salt (conductivity>16.0 mS/cm) and high organic matter content (COD>16,900 mg/kg). Therefore, the potential negative effects of the wastewater on soil salinity and pH should be assessed. 16S rRNA was used to investigate the bacterial population structure and assess potential biological risks of land use of the wastewater. The wastewater from lagoon has the least and the most bacterial abundance in summer and winter, respectively. The bacterial diversity of wastewater samples from lagoon is the most. 21 and 24 phyla were detected in winter and summer samples respectively. Clostridium is the absolute dominant bacteria in the summer water samples and the absolute dominant bacteria is Trichococcus in winter water samples. Several animal and plant bacterial pathogens such as Campylobacter, Corynebacterium, Facklamia and Erysipelothrix can be detected in the wastewater samples. More than 70% of pathogenic bacteria such as Campylobacter, Facklamia, Erysipelothrix and Acholeplasma can be removed by lagoon in summer, but only about 99% of Corynebacterium is still in the lagoon (XYH). While more than 60% of Corynebacterium, Facklamia and Erysipelothrix are not removed in winter. So the biological risk of land use of anaerobic pond wastewater needs to be considered.
为满足高密度聚乙烯(HDPE)膜封闭厌氧塘处理后废水的土地利用要求,测定了 HDPE 膜封闭厌氧塘中废水的理化性质。冬季和夏季膜封闭厌氧塘处理废水中的总氮(TN)含量分别为 3165 和 1510mg/L。废水可作为液体有机肥,部分替代化肥。废水对水稻的安全用量分别为 21.48t/ha 和玉米 9.54t/ha。同时,废水具有盐分高(电导率>16.0mS/cm)和有机物含量高(COD>16900mg/kg)的特点。因此,应对废水对土壤盐分和 pH 值的潜在负面影响进行评估。16S rRNA 用于研究细菌种群结构,并评估废水土地利用的潜在生物风险。夏季和冬季塘中废水的细菌丰度最低和最高。塘中废水样品的细菌多样性最高。冬季和夏季水样分别检测到 21 种和 24 种门。夏季水样中梭菌为绝对优势菌,冬季水样中绝对优势菌为毛球菌。废水中可检测到弯曲杆菌、棒状杆菌、法克氏菌和丹毒丝菌等几种动植物病原菌。Campylobacter、Facklamia、Erysipelothrix 和 Acholeplasma 等 70%以上的致病菌可在夏季通过塘去除,但只有约 99%的 Corynebacterium 仍留在塘中(XYH)。而冬季 Corynebacterium、Facklamia 和 Erysipelothrix 等超过 60%的菌不能去除。因此,需要考虑厌氧塘废水土地利用的生物风险。