Ouyang C H, Yeh H I, Huang T F
Toxicon. 1986;24(7):633-43. doi: 10.1016/0041-0101(86)90026-7.
A potent platelet aggregation inducer was purified from Calloselasma rhodostoma snake venom by Sephadex G-75, CM-Sephadex C-50 and Sephacryl S-300 column chromatography. It was homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with a molecular weight estimated to be 28,160 +/- 1280. It was devoid of phospholipase A2, fibrino(geno)lytic and thrombin-like activities. The venom inducer elicited platelet aggregation and the serotonin release reaction in rabbit platelet-rich plasma and platelet suspension. Exogenous calcium was required for its platelet activation. Creatine phosphate/creatine phosphokinase and indomethacin did not inhibit the venom inducer-induced aggregation and release reaction. Mepacrine and verapamil preferentially inhibited aggregation, while PGE1 completely blocked both aggregation and release reaction. It is concluded that the venom inducer activates platelets through the activation of endogenous phospholipase A2 or C, leading to intracellular calcium mobilization, but independent of the ADP release reaction or thromboxane A2 formation.
通过葡聚糖凝胶G - 75、CM - 葡聚糖凝胶C - 50和Sephacryl S - 300柱色谱法从圆斑蝰蛇毒中纯化出一种强效血小板聚集诱导剂。经十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳判断，它是均一的，估计分子量为28,160±1280。它缺乏磷脂酶A2、纤维蛋白（原）溶解和凝血酶样活性。该蛇毒诱导剂在富含兔血小板的血浆和血小板悬液中引发血小板聚集和5 - 羟色胺释放反应。其血小板激活需要外源性钙。磷酸肌酸/肌酸磷酸激酶和吲哚美辛不抑制蛇毒诱导剂引起的聚集和释放反应。米帕林和维拉帕米优先抑制聚集，而前列腺素E1完全阻断聚集和释放反应。得出的结论是，蛇毒诱导剂通过激活内源性磷脂酶A2或C来激活血小板，导致细胞内钙动员，但独立于ADP释放反应或血栓素A2形成。
