通过优化组合大肠杆菌 BL21 中的前体代谢模块，高效生产海藻糖。

Highly efficient production of ectoine via an optimized combination of precursor metabolic modules in Escherichia coli BL21.

机构信息

Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.

College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China.

出版信息

Bioresour Technol. 2023 Dec;390:129803. doi: 10.1016/j.biortech.2023.129803. Epub 2023 Sep 25.

DOI:10.1016/j.biortech.2023.129803

PMID:37758030

Abstract

Ectoine is an osmotic pressure protectant observed in various microorganisms and is widely used in cosmetics and pharmaceuticals. The market value of ectoine has increased considerably with social progress, resulting in high demand for ectoine production technology. Herein, a microbial cell factory in Escherichia coli that produces ectoine at high titers is described as developing efficient and environmentally friendly bio-based ectoine production technology. The ectoine biosynthetic pathway of Halomonas hydrothermalis was introduced into E. coli BL21 (DE3). Subsequent overexpression of precursor metabolic modules, including aspartate branching, pyruvate-oxoacetate, and glutamate biosynthesis pathways, resulted in the final strain, E. coli BCT08, which produced ectoine at a titer of 36.58 g/L during 30 h of fermentation. Sugar feeding speed optimization improved the ectoine titer to 131.8 g/L after 96 h of cultivation. This represents a remarkable achievement in ectoine production from glucose under low-salt conditions and has vast potential for industrial applications.

摘要

海藻糖是一种在各种微生物中发现的渗透压保护剂，广泛应用于化妆品和制药行业。随着社会的进步，海藻糖的市场价值大幅增长，导致对海藻糖生产技术的需求也很高。本文描述了一种在大肠杆菌中生产高浓度海藻糖的微生物细胞工厂，旨在开发高效、环保的基于生物的海藻糖生产技术。将来源于嗜盐古菌的海藻糖生物合成途径引入大肠杆菌 BL21（DE3）。随后过表达前体代谢模块，包括天冬氨酸分支、丙酮酸-氧代乙酸和谷氨酸生物合成途径，最终得到的菌株 E. coli BCT08 在 30 h 的发酵过程中生产 36.58 g/L 的海藻糖。糖进料速度优化后，在 96 h 的培养后，海藻糖的产量提高到 131.8 g/L。这是在低盐条件下从葡萄糖生产海藻糖的一项重大成就，具有广阔的工业应用潜力。