Coimbra André A B, Prakash Satya, Jiménez José I, Rios-Solis Leonardo
Department of Biochemical Engineering, Bernard Katz Building, University College London, Malet Pl, London, WC1E 6BT, UK.
Department of Life Sciences, Imperial College London, South Kensington, London, SW7 2AZ, UK.
Microb Cell Fact. 2025 Jun 12;24(1):133. doi: 10.1186/s12934-025-02757-2.
Halomonas species have recently emerged as promising chassis organisms for next-generation industrial biotechnology, due to their ability to thrive under high-salt conditions, where most microorganisms cannot survive. This feature minimizes contamination risks, thus enabling cultivation under open, unsterile conditions. In addition, many Halomonas species naturally produce large amounts of the bioplastic polyhydroxybutyrate and the high-value osmolyte ectoine.
This review explores the development of genetic manipulation tools and their pivotal role in establishing the genus Halomonas as an industrial chassis. Key additions to the synthetic biology toolbox, including cloning vectors, genetic parts, and genome editing systems are highlighted, along with challenges faced for their adoption, such as difficulties in transformation. In addition, we showcase how these tools have been employed for the development of more robust, high-producing strains through metabolic engineering, as well as for expanding the portfolio of target metabolites produced by Halomonas.
Recent developments in synthetic biology tools and metabolic engineering highlighted in this review underscore the potential of Halomonas for large scale metabolite production and provide a promising outlook towards their role as a microbial chassis in industrial biotechnology.
由于嗜盐单胞菌属能够在大多数微生物无法存活的高盐条件下茁壮生长，它们最近已成为下一代工业生物技术中颇具潜力的底盘生物。这一特性将污染风险降至最低，从而能够在开放的非无菌条件下进行培养。此外，许多嗜盐单胞菌属天然会产生大量的生物塑料聚羟基丁酸酯和高价值的渗透保护剂四氢嘧啶。
本综述探讨了基因操作工具的发展及其在将嗜盐单胞菌属确立为工业底盘方面的关键作用。重点介绍了合成生物学工具包的关键新增内容，包括克隆载体、遗传元件和基因组编辑系统，以及采用这些工具时面临的挑战，如转化困难。此外，我们展示了如何通过代谢工程利用这些工具开发更强壮、高产的菌株，以及扩大嗜盐单胞菌属产生的目标代谢物组合。
本综述中强调的合成生物学工具和代谢工程的最新进展突出了嗜盐单胞菌属在大规模代谢物生产方面的潜力，并为其在工业生物技术中作为微生物底盘的作用提供了充满希望的前景。
