State Key Laboratory of Trauma, Burn and Combined Injury, Third Military Medical University, Chongqing 400038, China; Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 4):127015. doi: 10.1016/j.ijbiomac.2023.127015. Epub 2023 Sep 25.
Peripheral nerve injuries (PNI) currently have limited therapeutic efficacy, and functional scaffolds have been shown to be effective for treating PNI. Ovalbumin (OVA) is widely used as a natural biomaterial for repairing damaged tissues due to its excellent biocompatibility and the presence of various bioactive components. However, there are few reports on the repair of PNI by ovalbumin. In this study, a novel bionic functionalized topological scaffold based on ovalbumin and grafted with tyrosine-isoleucine-glycine-serine-arginine (YIGSR) peptide was constructed by micro-molding method and surface-biomodification technology. The scaffolds were subjected to a series of evaluations in terms of morphology, mechanics, hydrophilicity, and biocompatibility, and the related molecular mechanisms were further penetrated. The results showed that the scaffolds prepared in this study had aligned ridge/groove structure, good mechanical properties and biocompatibility, and could be used as carriers to slowly release YIGSR, which effectively promoted the proliferation, migration and elongation of Schwann Cells (SCs), and significantly up-regulated the gene expression related to proliferation, apoptosis, migration and axon regeneration. Therefore, the bionic functional topological scaffold has significant application potential for promoting peripheral nerve regeneration and provides a new therapeutic option for repairing PNI.
周围神经损伤（PNI）目前的治疗效果有限，而功能支架已被证明对 PNI 的治疗有效。卵清蛋白（OVA）由于其优异的生物相容性和存在各种生物活性成分，被广泛用作修复受损组织的天然生物材料。然而，关于卵清蛋白修复 PNI 的报道很少。在本研究中，通过微成型方法和表面生物修饰技术构建了一种新型仿生功能化拓扑支架，该支架基于卵清蛋白并接枝了酪氨酸-异亮氨酸-甘氨酸-丝氨酸-精氨酸（YIGSR）肽。对支架进行了形态学、力学、亲水性和生物相容性等一系列评价，并进一步研究了相关的分子机制。结果表明，本研究制备的支架具有规则的脊/槽结构，具有良好的机械性能和生物相容性，可用作载体缓慢释放 YIGSR，有效促进雪旺细胞（SCs）的增殖、迁移和伸长，并显著上调与增殖、凋亡、迁移和轴突再生相关的基因表达。因此，仿生功能拓扑支架在促进周围神经再生方面具有重要的应用潜力，为修复 PNI 提供了新的治疗选择。
