Wasserman M, Beltrán R M, Quintana F O, Mendoza P M, Orozco L C, Rodriguez G
Tubercle. 1986 Jun;67(2):83-90. doi: 10.1016/0041-3879(86)90001-2.
A method for the preparation of liposomes loaded with rifampicin and isoniazid is described. Optimal conditions were established; the lipid suspension was mixed with the aqueous solution of the drugs and was sonicated in a bath for 30 min at 50 degrees C. The optimum composition tested was phosphatidyl choline, cholesterol and cardiolipin in a molar ratio of 7:2:1. The separation of unloaded drug was performed by centrifugation through three successive Sephadex G-25 columns. The liposomes were multilamellar vesicles with a size ranging from 100-300 nm. The drugs were trapped in concentrations from 6.5-9.5 mg/ml. This method is suitable for preparation of liposomes in small laboratories.
描述了一种制备负载利福平和异烟肼的脂质体的方法。确定了最佳条件；将脂质悬浮液与药物水溶液混合，并在50℃的水浴中超声处理30分钟。测试的最佳组成为摩尔比为7:2:1的磷脂酰胆碱、胆固醇和心磷脂。通过连续通过三个葡聚糖G-25柱进行离心来分离未负载的药物。脂质体为多层囊泡，大小范围为100 - 300nm。药物的包封浓度为6.5 - 9.5mg/ml。该方法适用于小型实验室制备脂质体。
