Wang Xumiao, Hu Wei, Li Yuxi, Jiang Minghao, Zhao Ning, Cao Haiqun, Liao Min
Anhui Province Key Laboratory of Crop Integrated Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China.
Anhui Province Engineering Laboratory for Green Pesticide Development and Application, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China.
Biology (Basel). 2023 Aug 31;12(9):1192. doi: 10.3390/biology12091192.
Large crabgrass ( (L.) Scop.) is one of the major malignant grass weeds in Chinese maize ( L.) fields, and it has recently developed resistance to the acetolactate synthase (ALS)-inhibiting herbicide nicosulfuron. This study focused on a suspected nicosulfuron-resistant (R) population (LJ-01) of , collected from Lujiang County in Anhui Province, China, to explore the resistance level and potential resistance mechanism. Whole-plant dose-response testing confirmed that the LJ-01 population evolved a high level of resistance to nicosulfuron (11.5-fold) compared to the susceptible (S) population, DY-02. The gene sequencing and relative expression assay of the R plants indicated that target gene mutation and overexpression were not responsible for the resistance phenotype. However, pretreatment with malathion, a known cytochrome P450 monooxygenase (P450) inhibitor, alleviated the resistance of the R population to nicosulfuron by approximately 36%. High-performance liquid chromatography (HPLC) analysis revealed that the R plants metabolized nicosulfuron faster than the S plants. Moreover, cross-resistance testing suggested that the R population exhibited low levels of resistance to thifensulfuron-methyl and pyrazosulfuron-ethyl, but it remained susceptible to rimsulfuron. Multiple resistance patterns showed that the R population evolved low resistance to the photosystem inhibitors bromoxynil octanoate and atrazine and sensitivity to the acetyl-CoA carboxylase (ACCase) inhibitor cyhalofop-butyl and the 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors tembotrione, mesotrione, and topramezone. This study reports, for the first time, the simultaneous resistance to ALS and different photosystem inhibitors in . The nicosulfuron resistance observed in the R population could primarily be attributed to an enhanced metabolism involving P450 enzymes.
大狗尾草（Setaria faberi (L.) Scop.）是中国玉米（Zea mays L.）田中的主要恶性杂草之一，且最近已对乙酰乳酸合成酶（ALS）抑制型除草剂烟嘧磺隆产生抗性。本研究聚焦于从中国安徽省庐江县采集的一个疑似对烟嘧磺隆具有抗性（R）的大狗尾草种群（LJ - 01），以探究其抗性水平及潜在抗性机制。整株剂量反应测试证实，与敏感（S）种群DY - 02相比，LJ - 01种群对烟嘧磺隆进化出了高水平抗性（11.5倍）。对R植株的ALS基因测序及相对表达分析表明，靶基因突变和过表达并非导致抗性表型的原因。然而，用已知的细胞色素P450单加氧酶（P450）抑制剂马拉硫磷预处理，可使R种群对烟嘧磺隆的抗性降低约36%。高效液相色谱（HPLC）分析显示，R植株比S植株更快地代谢烟嘧磺隆。此外，交互抗性测试表明，R种群对甲基硫菌灵和苄嘧磺隆表现出低水平抗性，但对砜嘧磺隆仍敏感。多重抗性模式表明，R种群对光系统抑制剂辛酸溴苯腈和莠去津进化出低抗性，对乙酰辅酶A羧化酶（ACCase）抑制剂氰氟草酯以及4 - 羟苯基丙酮酸双加氧酶（HPPD）抑制剂苯唑草酮、甲基磺草酮和硝磺草酮敏感。本研究首次报道了大狗尾草对ALS和不同光系统抑制剂的同时抗性。在R种群中观察到的烟嘧磺隆抗性可能主要归因于涉及P450酶的代谢增强。
