Sánchez-Somolinos Mar, Díaz-Navarro Marta, Benjumea Antonio, Matas José, Vaquero Javier, Muñoz Patricia, Sanz-Ruíz Pablo, Guembe María
Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, 28007 Madrid, Spain.
Instituto de Investigación Sanitaria Gregorio Marañón, 28007 Madrid, Spain.
Antibiotics (Basel). 2023 Sep 13;12(9):1445. doi: 10.3390/antibiotics12091445.
Based on previous studies by our group in which we demonstrated that dalbavancin loaded in bone cement had good elution capacity for the treatment of biofilm-related periprosthetic infections, we now assess the anti-biofilm activity of dalbavancin and compare it with that of vancomycin over a 3-month period. We designed an in vitro model in which we calculated the percentage reduction in log cfu/mL counts of sonicated steel discs contaminated with staphylococci and further exposed to bone cement discs loaded with 2.5% or 5% vancomycin and dalbavancin at various timepoints (24 h, 48 h, 1 week, 2 weeks, 6 weeks, and 3 months). In addition, we tested the anti-biofilm activity of eluted vancomycin and dalbavancin at each timepoint based on a 96-well plate model in which we assessed the percentage reduction in metabolic activity. We observed a significant decrease in the dalbavancin concentration from 2 weeks of incubation, with sustained anti-biofilm activity up to 3 months. In the case of vancomycin, we observed a significant decrease at 1 week. The concentration gradually increased, leading to significantly lower anti-biofilm activity. The percentage reduction in cfu/mL counts was higher for dalbavancin than for vancomycin at both the 2.5% and the 5% concentrations. The reduction in log cfu/mL counts was higher for than for and was particularly more notable for 5% dalbavancin at 3 months. In addition, the percentage reduction in metabolic activity also decreased at 3 months in 5% dalbavancin and 5% vancomycin, with more notable values recorded for the latter.
基于我们团队之前的研究，我们证明了载于骨水泥中的达巴万星对生物膜相关的假体周围感染具有良好的洗脱能力，现在我们评估达巴万星的抗生物膜活性，并在3个月的时间内将其与万古霉素的抗生物膜活性进行比较。我们设计了一个体外模型，计算被葡萄球菌污染并在不同时间点（24小时、48小时、1周、2周、6周和3个月）进一步暴露于载有2.5%或5%万古霉素和达巴万星的骨水泥盘的超声处理钢盘的log cfu/mL计数的减少百分比。此外，我们基于96孔板模型在每个时间点测试洗脱的万古霉素和达巴万星的抗生物膜活性，在该模型中我们评估代谢活性的减少百分比。我们观察到达巴万星浓度从培养2周起显著下降，直至3个月都保持持续的抗生物膜活性。对于万古霉素，我们观察到在1周时显著下降。其浓度逐渐增加，导致抗生物膜活性显著降低。在2.5%和5%浓度下，达巴万星的cfu/mL计数减少百分比均高于万古霉素。对于[此处原文缺失比较对象]，log cfu/mL计数的减少高于[此处原文缺失比较对象]，在3个月时5%达巴万星的情况尤为明显。此外，5%达巴万星和5%万古霉素在3个月时代谢活性的减少百分比也下降，后者记录的值更显著。
