Maurer Sost Mônica, Stevens Yala, Salden Bouke, Troost Freddy, Masclee Ad, Venema Koen
Centre for Healthy Eating & Food Innovation (HEFI), Maastricht University-Campus Venlo, 5928 SZ Venlo, The Netherlands.
BioActor BV, 6229 GS Maastricht, The Netherlands.
Foods. 2023 Sep 13;12(18):3413. doi: 10.3390/foods12183413.
The objective of this study was to investigate the effects of a citrus extract rich in citrus flavonoids on intestinal metabolic responses in subjects with features of metabolic syndrome, in an in vitro colon fermentation system (TIM-2) and fecal samples obtained from human subjects in an in vivo trial. In the TIM-2 system inoculated with fecal samples of volunteers with features of metabolic syndrome, continuous citrus extract supplementation (500 mg/day) resulted in increased cumulative short-chain fatty acid (SCFA) levels compared to the control condition, which was mainly due to increased production of butyrate, acetate, and valerate. In human volunteers, 12 weeks of daily supplementation with 500 mg citrus extract resulted in a significant shift in the SCFA profile towards more butyrate ( = 0.022) compared to the placebo group. Furthermore, there was a trend towards a reduction in fecal calprotectin levels, a marker for intestinal inflammation, compared to the placebo ( = 0.058). Together, these results suggest that citrus extract intake may have a positive effect on intestinal metabolic responses and through this, on host health in subjects with features of metabolic syndrome. Further research is needed to provide more insight into the potential underlying mechanisms and to study effects on clinical parameters.
本研究的目的是在体外结肠发酵系统（TIM-2）以及从人体受试者体内试验获得的粪便样本中，研究富含柑橘类黄酮的柑橘提取物对具有代谢综合征特征的受试者肠道代谢反应的影响。在接种了具有代谢综合征特征志愿者粪便样本的TIM-2系统中，与对照条件相比，持续补充柑橘提取物（500毫克/天）导致累积短链脂肪酸（SCFA）水平升高，这主要是由于丁酸盐、乙酸盐和戊酸盐的产生增加。在人体志愿者中，与安慰剂组相比，每天补充500毫克柑橘提取物12周导致SCFA谱显著向更多丁酸盐方向转变（P = 0.022）。此外，与安慰剂相比，粪便钙卫蛋白水平（肠道炎症标志物）有降低趋势（P = 0.058）。总之，这些结果表明，摄入柑橘提取物可能对肠道代谢反应有积极影响，并由此对具有代谢综合征特征的受试者的宿主健康产生积极影响。需要进一步研究以更深入了解潜在的机制，并研究其对临床参数的影响。
