Chen Li, Li Ke, Chen Huitai, Li Zongjun
College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Hunan Guoyuan Liquor Industry Co., Ltd., Yueyang 414000, China.
Foods. 2023 Sep 20;12(18):3501. doi: 10.3390/foods12183501.
Flavor is an essential element of food quality. Flavor can be improved by adding flavoring substances or via microbial fermentation to impart aroma. Aroma-producing yeasts are a group of microorganisms that can produce aroma compounds, providing a strong aroma to foods and thus playing a great role in the modern fermentation industry. The physiological characteristics of aroma-producing yeast, including alcohol tolerance, acid tolerance, and salt tolerance, are introduced in this article, beginning with their origins and biological properties. The main mechanism of aroma-producing yeast is then analyzed based on its physiological roles in the fermentation process. Functional enzymes such as proteases, lipases, and glycosidase are released by yeast during the fermentation process. Sugars, fats, and proteins in the environment can be degraded by these enzymes via pathways such as glycolysis, methoxylation, the Ehrlich pathway, and esterification, resulting in the production of various aromatic esters (such as ethyl acetate and ethyl caproate), alcohols (such as phenethyl alcohol), and terpenes (such as monoterpenes, sesquiterpenes, and squalene). Furthermore, yeast cells can serve as cell synthesis factories, wherein specific synthesis pathways can be introduced into cells using synthetic biology techniques to achieve high-throughput production. In addition, the applications of aroma yeast in the food, pharmaceutical, and cosmetic industries are summarized, and the future development trends of aroma yeasts are discussed to provide a theoretical basis for their application in the food fermentation industry.
风味是食品品质的重要要素。可通过添加调味物质或借助微生物发酵来赋予香气,从而改善风味。产香酵母是一类能够产生香气化合物的微生物,可为食品提供浓郁香气,因此在现代发酵工业中发挥着重要作用。本文从产香酵母的起源和生物学特性入手,介绍了其生理特性,包括耐酒精性、耐酸性和耐盐性。随后基于其在发酵过程中的生理作用,分析了产香酵母的主要作用机制。酵母在发酵过程中会释放蛋白酶、脂肪酶和糖苷酶等功能酶。环境中的糖类、脂肪和蛋白质可通过这些酶经糖酵解、甲氧基化、埃利希途径和酯化等途径降解,从而产生各种芳香酯(如乙酸乙酯和己酸乙酯)、醇类(如苯乙醇)和萜类(如单萜、倍半萜和角鲨烯)。此外,酵母细胞可作为细胞合成工厂,利用合成生物学技术将特定合成途径引入细胞,以实现高通量生产。此外,还总结了产香酵母在食品、制药和化妆品行业的应用,并探讨了产香酵母的未来发展趋势,为其在食品发酵工业中的应用提供理论依据。