Cao Jiafeng, Kong Weiguang, Cheng Gaofeng, Xu Zhen
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Int J Mol Sci. 2023 Sep 6;24(18):13745. doi: 10.3390/ijms241813745.
Granulocytes are crucial innate immune cells that have been extensively studied in teleost fish. Studies in mammals have revealed that mechanistic target of rapamycin complex 1 (mTORC1) signaling acts as a significant immune regulatory hub, influencing granulocyte immune function. To investigate whether mTORC1 signaling also regulates the immune function of granulocytes in teleost fish, we established a model of RAPA inhibition of the mTORC1 signaling pathway using granulocytes from largemouth bass (). Our results demonstrated that inhibition of mTORC1 signaling promoted autophagy and apoptosis of granulocytes while inhibiting cell proliferation. Moreover, inhibition of the mTORC1 signaling pathway enhanced the phagocytosis capacity of granulocytes. Collectively, our findings revealed the evolutionarily conserved role of the mTORC1 signaling pathway in regulating granulocyte responses, thus providing novel insights into the function of granulocytes in teleost fish.
粒细胞是硬骨鱼中已被广泛研究的关键先天性免疫细胞。哺乳动物研究表明，雷帕霉素复合物1（mTORC1）信号传导作为重要的免疫调节枢纽，影响粒细胞免疫功能。为研究mTORC1信号传导是否也调节硬骨鱼中粒细胞的免疫功能，我们利用大口黑鲈的粒细胞建立了雷帕霉素（RAPA）抑制mTORC1信号通路的模型。我们的结果表明，抑制mTORC1信号传导可促进粒细胞的自噬和凋亡，同时抑制细胞增殖。此外，抑制mTORC1信号通路可增强粒细胞的吞噬能力。总体而言，我们的研究结果揭示了mTORC1信号通路在调节粒细胞反应中进化上保守的作用，从而为硬骨鱼中粒细胞的功能提供了新见解。
