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在 和 中的克隆及其功能分析。

Cloning of in and an Analysis of Its Function.

机构信息

State Key Laboratory of Tree Genetics and Breeding, Key Open Laboratory of Forest Genetics and Gene Engineering of National Forestry & Grassland Administration, Key Laboratory of Forestry Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.

出版信息

Int J Mol Sci. 2023 Sep 6;24(18):13766. doi: 10.3390/ijms241813766.

Abstract

Phenylpropanoids are crucial for the growth and development of plants and their interaction with the environment. As key transcriptional regulators of plant growth and development, MYB-like transcription factors play a vital role in the biosynthesis of phenylpropanoid metabolites. In this study, we functionally characterized , a gene that encodes an R2R3-MYB transcription factor. It was confirmed by qPCR that was highly expressed in the flowers, xylem, and phloem of . By overexpressing in tobacco and poplar, we found that transgenic plants had enlarged xylem, increased content of lignin and flavonoids, and up-regulated expression of several enzyme genes of the phenylpropane metabolism pathway to different degrees. The above research results indicate that is involved in the metabolic flux distribution of different branches of the phenylpropane metabolic pathway, and the results may provide clues for the regulation of metabolic fluxes between flavonoids and the lignin biosynthesis pathways of , as well as provide a basis for the molecular breeding of .

摘要

苯丙烷类化合物对于植物的生长发育及其与环境的相互作用至关重要。作为植物生长发育的关键转录调控因子,MYB 类转录因子在苯丙烷类代谢物的生物合成中发挥着重要作用。本研究中,我们对一个编码 R2R3-MYB 转录因子的基因 进行了功能表征。qPCR 结果证实, 在 中的花、木质部和韧皮部中高度表达。通过在烟草和杨树中过表达 ,我们发现转基因植物的木质部增大,木质素和类黄酮含量增加,并且苯丙烷代谢途径中的几个酶基因的表达水平也不同程度地上调。上述研究结果表明, 参与了苯丙烷代谢途径不同分支的代谢通量分配,这一结果可能为调控黄酮类化合物和木质素生物合成途径之间的代谢通量提供线索,同时也为 分子育种提供了依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ab/10530544/b08259f0c892/ijms-24-13766-g001.jpg

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