Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Research Center of the Basic Discipline Cell Biology, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
Int J Mol Sci. 2023 Sep 8;24(18):13839. doi: 10.3390/ijms241813839.
Leaf senescence is an important factor affecting the functional transition from nutrient assimilation to nutrient remobilization in crops. The senescence of wheat leaves is of great significance for its yield and quality. In the leaf senescence process, transcriptional regulation is a committed step in integrating various senescence-related signals. Although the plant-specific transcriptional regulation factor valine-glutamine (VQ) gene family is known to participate in different physiological processes, its role in leaf senescence is poorly understood. We isolated TaVQ25-A and studied its function in leaf senescence regulation. - was mainly expressed in the roots and leaves of wheat. The TaVQ25-A-GFP fusion protein was localized in the nuclei and cytoplasm of wheat protoplasts. A delayed senescence phenotype was observed after dark and abscisic acid (ABA) treatment in --silenced wheat plants. Conversely, overexpression of - accelerated leaf senescence and led to hypersensitivity in ABA-induced leaf senescence in . A WRKY type transcription factor, TaWRKY133, which is tightly related to the ABA pathway and affects the expression of some ABA-related genes, was found to interact with TaVQ25-A both in vitro and in vivo. Results of this study indicate that TaVQ25-A is a positive regulator of ABA-related leaf senescence and can be used as a candidate gene for wheat molecular breeding.
叶片衰老是影响作物从养分吸收到养分再利用的功能转变的重要因素。小麦叶片的衰老对其产量和品质具有重要意义。在叶片衰老过程中，转录调控是整合各种衰老相关信号的关键步骤。尽管植物特异性转录调控因子缬氨酸-谷氨酰胺（VQ）基因家族已知参与不同的生理过程，但它在叶片衰老中的作用仍知之甚少。我们分离了 TaVQ25-A，并研究了它在叶片衰老调控中的功能。TaVQ25-A 在小麦的根和叶中主要表达。TaVQ25-A-GFP 融合蛋白定位于小麦原生质体的细胞核和细胞质中。在沉默小麦植株中，经黑暗和脱落酸（ABA）处理后，观察到衰老延迟表型。相反，过表达 - 加速叶片衰老，并导致在 ABA 诱导的叶片衰老中表现出超敏性。TaWRKY133 是一种与 ABA 途径紧密相关并影响一些 ABA 相关基因表达的 WRKY 型转录因子，被发现与 TaVQ25-A 在体外和体内均相互作用。这项研究的结果表明，TaVQ25-A 是 ABA 相关叶片衰老的正调节剂，可作为小麦分子育种的候选基因。
