Department of Biochemistry, National Defense Medical Center, Taipei 114, Taiwan.
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan.
Int J Mol Sci. 2023 Sep 14;24(18):14081. doi: 10.3390/ijms241814081.
Urothelial cancer, a common urinary system malignancy, often presents treatment challenges due to metastasis and chemotherapy side effects. Angiogenesis, crucial for tumor growth, has become a target for drug development. This study explores the expression, prognostic value, and clinical correlation of in the TCGA BLCA, GSE31684, and GSE32894 datasets. We identify common differentially expressed genes across these databases and utilize g:Profiler and Cytoscape ClueGO for functional assessment. Further, we perform a gene set enrichment analysis (GSEA) using Hallmark gene sets and use the imsig package for immune cell infiltration analysis. Our analysis indicates that expression levels significantly impact survival rates, tumor progression, and immune response in urothelial tumors. High expression correlated with poor prognosis, advanced disease stages, and an increase in monocyte population within the tumor microenvironment. This aligns with current literature indicating a key role of immune infiltration in bladder cancer progression and treatment response. Moreover, the GSEA and imsig results further suggest a potential mechanistic link between expression and immune-related pathways. Considering the increasing emphasis on immunotherapeutic strategies in bladder cancer management, our findings on 's potential as a diagnostic biomarker and its association with immune response open new avenues for therapeutic interventions.
膀胱癌是一种常见的泌尿系统恶性肿瘤，由于转移和化疗副作用，常带来治疗挑战。血管生成对于肿瘤生长至关重要，已成为药物开发的靶点。本研究通过 TCGA BLCA、GSE31684 和 GSE32894 数据集，探讨了 在其中的表达、预后价值和临床相关性。我们确定了这些数据库中常见的差异表达基因，并使用 g:Profiler 和 Cytoscape ClueGO 进行功能评估。此外，我们使用 Hallmark 基因集进行基因集富集分析（GSEA），并使用 imsig 包进行免疫细胞浸润分析。我们的分析表明， 在尿路上皮肿瘤中的表达水平显著影响生存率、肿瘤进展和免疫反应。高 表达与预后不良、疾病进展期和肿瘤微环境中单核细胞群体增加相关。这与当前文献表明免疫浸润在膀胱癌进展和治疗反应中的关键作用一致。此外，GSEA 和 imsig 结果进一步表明 表达与免疫相关途径之间存在潜在的机制联系。鉴于免疫治疗策略在膀胱癌管理中的重要性日益增加，我们关于 '的发现作为一种诊断生物标志物的潜力及其与免疫反应的关联为治疗干预开辟了新途径。
