Jang Yeongseok, Jung Jinmu, Oh Jonghyun
Department of Mechanical Design Engineering, Jeonbuk National University, Jeonju-si 54896, Jeollabuk-do, Republic of Korea.
Department of Nano-Bio Mechanical System Engineering, Jeonbuk National University, Jeonju-si 54896, Jeollabuk-do, Republic of Korea.
Micromachines (Basel). 2023 Sep 4;14(9):1736. doi: 10.3390/mi14091736.
Traditional goal of microfabrication was to limitedly construct nano- and micro-geometries on silicon or quartz wafers using various semiconductor manufacturing technologies, such as photolithography, soft lithography, etching, deposition, and so on. However, recent integration with biotechnologies has led to a wide expansion of microfabrication. In particular, many researchers studying pharmacology and pathology are very interested in producing in vitro models that mimic the actual intestine to study the effectiveness of new drug testing and interactions between organs. Various bio-microfabrication techniques have been developed while solving inherent problems when developing in vitro micromodels that mimic the real large intestine. This intensive review introduces various bio-microfabrication techniques that have been used, until recently, to realize two-dimensional and three-dimensional biomimetic experimental models. Regarding the topic of gut chips, two major review subtopics and two-dimensional and three-dimensional gut chips were employed, focusing on the membrane-based manufacturing process for two-dimensional gut chips and the scaffold-based manufacturing process for three-dimensional gut chips, respectively.
传统的微纳加工目标是利用各种半导体制造技术，如光刻、软光刻、蚀刻、沉积等，在硅或石英晶圆上有限地构建纳米和微米级几何结构。然而，最近与生物技术的整合导致了微纳加工的广泛扩展。特别是，许多研究药理学和病理学的研究人员对构建模拟实际肠道的体外模型非常感兴趣，以研究新药测试的有效性以及器官之间的相互作用。在开发模拟真实大肠的体外微模型时，人们在解决固有问题的同时，开发了各种生物微纳加工技术。这篇深入综述介绍了直到最近还被用于实现二维和三维仿生实验模型的各种生物微纳加工技术。关于肠道芯片这一主题，采用了两个主要的综述子主题，即二维和三维肠道芯片，分别重点介绍了基于膜的二维肠道芯片制造工艺和基于支架的三维肠道芯片制造工艺。
