Wang Zhaochun, Liu Jian, Xu Haiyan, Liu Jiating, Zhao Zhiwei, Gong Xun
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, China.
Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Microorganisms. 2023 Aug 24;11(9):2144. doi: 10.3390/microorganisms11092144.
Endophytes are essential in plant succession and evolution, and essential for stress resistance. Coralloid root is a unique root structure found in cycads that has played a role in resisting adverse environments, yet the core taxa and microbial community of different species have not been thoroughly investigated. Using amplicon sequencing, we successfully elucidated the microbiomes present in coralloid roots of 10 species, representing all four sections of in China. We found that the endophytic bacteria in coralloid roots, i.e., , were mainly composed of _PCC-7422, _PCC-73102 and . Additionally, the Ascomycota fungi of , , , , , and were identified as the core fungi taxa. The Ascomycota fungi of Nectriaceae, Herpotrichiellaceae, Cordycipitaceae, Helotiaceae, Diaporthaceae, Didymellaceae, Clavicipitaceae and Pleosporaceae were identified as the core family taxa in coralloid roots of four sections. High abundance but low diversity of bacterial community was detected in the coralloid roots, but no significant difference among species. The fungal community exhibited much higher complexity compared to bacteria, and diversity was noted among different species or sections. These core taxa, which were a subset of the microbiome that frequently occurred in all, or most, individuals of species, represent targets for the development of conservation.
内生菌在植物演替和进化中至关重要，对植物抗逆性也必不可少。珊瑚状根是苏铁类植物中一种独特的根系结构，在抵抗不利环境中发挥了作用，但不同物种的核心分类群和微生物群落尚未得到充分研究。利用扩增子测序，我们成功阐明了代表中国所有四个组的10个物种的珊瑚状根中存在的微生物群落。我们发现，珊瑚状根中的内生细菌主要由_PCC - 7422、PCC - 73102和_组成。此外，、、、_、_和_的子囊菌门真菌被鉴定为核心真菌分类群。肉座菌科、毛壳菌科、虫草科、锤舌菌科、间座壳科、座囊菌科、麦角菌科和格孢腔菌科的子囊菌门真菌被鉴定为四个组的珊瑚状根中的核心科分类群。在珊瑚状根中检测到细菌群落丰度高但多样性低，且不同物种间无显著差异。真菌群落比细菌群落表现出更高的复杂性，不同物种或组之间存在多样性。这些核心分类群是微生物群落的一个子集，经常出现在一个物种的所有或大多数个体中，是苏铁保护发展的目标。
