Aslam Muhammad, Pei Pengbing, Ye Peilin, Li Tangcheng, Liang Honghao, Zhang Zezhi, Ke Xiao, Chen Weizhou, Du Hong
Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, College of Science, Shantou University, Shantou 515063, China.
Faculty of Marine Sciences, Lasbela University of Agriculture, Water and Marine Sciences, Uthal 90150, Pakistan.
Microorganisms. 2023 Sep 4;11(9):2228. doi: 10.3390/microorganisms11092228.
N-acyl homoserine lactones (AHLs) are small, diffusible chemical signal molecules that serve as social interaction tools for bacteria, enabling them to synchronize their collective actions in a density-dependent manner through quorum sensing (QS). The QS activity from epiphytic bacteria of the red macroalgae , along with its involvement in biofilm formation and regulation, remains unexplored in prior scientific inquiries. Therefore, this study explores the AHL signal molecules produced by epiphytic bacteria. The bacterium isolated from the surface of was identified as by 16s rRNA gene sequencing and screened for AHLs using two AHL reporter strains, A136 and CV026. The crystal violet assay was used for the biofilm-forming phenotype. The inferences revealed that produces four different types of AHL molecules, i.e., C4-HSL, C8-HSL, C18-HSL, and 3-oxo-C16-HSL, and it was observed that its biofilm formation phenotype is regulated by QS molecules. This is the first study providing insights into the QS activity, diverse AHL profile, and regulatory mechanisms that govern the biofilm formation phenotype of . These findings offer valuable insights for future investigations exploring the role of AHL producing epiphytes and biofilms in the life cycle of .
N-酰基高丝氨酸内酯（AHLs）是一类可扩散的小分子化学信号分子，作为细菌的社交互动工具，使它们能够通过群体感应（QS）以密度依赖的方式同步其集体行为。红藻附生细菌的群体感应活性及其在生物膜形成和调控中的作用，在以往的科学研究中尚未得到探索。因此，本研究对附生细菌产生的AHL信号分子进行了探索。通过16s rRNA基因测序，从[具体红藻名称未给出]表面分离出的细菌被鉴定为[具体细菌名称未给出]，并使用两种AHL报告菌株A136和CV026对其进行AHL筛选。采用结晶紫测定法研究生物膜形成表型。推断结果表明，[具体细菌名称未给出]产生四种不同类型的AHL分子，即C4-HSL、C8-HSL、C18-HSL和3-氧代-C16-HSL，并且观察到其生物膜形成表型受QS分子调控。这是第一项深入研究[具体细菌名称未给出]的群体感应活性、多样的AHL谱以及调控生物膜形成表型的机制的研究。这些发现为未来探索产生AHL的附生植物和生物膜在[具体红藻名称未给出]生命周期中的作用的研究提供了有价值的见解。
