qPCR-Based Monitoring of 2-Methylisoborneol/Geosmin-Producing Cyanobacteria in Drinking Water Reservoirs in South Korea.

Cyanobacteria can exist in water resources and produce odorants. 2-Methylisoborneol (2-MIB) and geosmin are the main odorant compounds affecting the drinking water quality in reservoirs. In this study, encoding genes 2-MIB (, ) and geosmin (, ) were investigated using newly developed primers for quantitative PCR (qPCR). Gene copy numbers were compared to 2-MIB/geosmin concentrations and cyanobacterial cell abundance. Samples were collected between July and October 2020, from four drinking water sites in South Korea. The results showed similar trends in three parameters, although the changes in the 2-MIB/geosmin concentrations followed the changes in the / copy numbers more closely than the cyanobacterial cell abundances. The number of odorant gene copies decreased from upstream to downstream. Regression analysis revealed a strong positive linear correlation between gene copy number and odorant concentration for ( = 0.8478) and ( = 0.601). In the analysis of several environmental parameters, only water temperature was positively correlated with both and . Our results demonstrated the feasibility of monitoring 2-MIB/geosmin occurrence using qPCR of their respective synthase genes. Odorant-producing, gene-based qPCR monitoring studies may contribute to improving drinking water quality management.