College of Bioengineering, Beijing Polytechnic, Beijing 100176, China.
Molecules. 2023 Sep 21;28(18):6745. doi: 10.3390/molecules28186745.
The functional pool of canonical amino acids (cAAs) has been enriched through the emergence of non-canonical amino acids (ncAAs). NcAAs play a crucial role in the production of various pharmaceuticals. The biosynthesis of ncAAs has emerged as an alternative to traditional chemical synthesis due to its environmental friendliness and high efficiency. The breakthrough genetic code expansion (GCE) technique developed in recent years has allowed the incorporation of ncAAs into target proteins, giving them special functions and biological activities. The biosynthesis of ncAAs and their incorporation into target proteins within a single microbe has become an enticing application of such molecules. Based on that, in this study, we first review the biosynthesis methods for ncAAs and analyze the difficulties related to biosynthesis. We then summarize the GCE methods and analyze their advantages and disadvantages. Further, we review the application progress of ncAAs and anticipate the challenges and future development directions of ncAAs.
天然存在的氨基酸（cAAs）功能库已通过非天然氨基酸（ncAAs）的出现得到了丰富。ncAAs 在各种药物的生产中起着至关重要的作用。由于其环境友好性和高效率，ncAAs 的生物合成已经成为传统化学合成的替代方法。近年来开发的突破性遗传密码扩展（GCE）技术允许将 ncAAs 掺入目标蛋白中，赋予它们特殊的功能和生物学活性。ncAAs 的生物合成及其在单个微生物内掺入目标蛋白已成为此类分子的诱人应用。基于此，在本研究中，我们首先综述了 ncAAs 的生物合成方法，并分析了与生物合成相关的困难。然后我们总结了 GCE 方法，并分析了它们的优缺点。此外，我们综述了 ncAAs 的应用进展，并对 ncAAs 的挑战和未来发展方向进行了预测。
Zotero 插件
