• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

绿藻室内和室外培养的有效pH值和溶解二氧化碳条件

Efficient pH and dissolved CO conditions for indoor and outdoor cultures of green alga .

作者信息

Takagi Akari, Nagao Misato, Uejima Yuya, Sasaki Daisaku, Asayama Munehiko

机构信息

College of Agriculture, Ibaraki University, Ibaraki, Japan.

BioX Chemical Industries Co., Ltd., Hiroshima, Japan.

出版信息

Front Bioeng Biotechnol. 2023 Sep 11;11:1233944. doi: 10.3389/fbioe.2023.1233944. eCollection 2023.

DOI:10.3389/fbioe.2023.1233944
PMID:37767110
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10520278/
Abstract

Efficient pH and dissolved CO conditions for indoor (50-450 mL scale) and outdoor (100-500 L scale) culture of a green alga BX1.5 strain that can produce useful intracellular lipids and extracellular polysaccharides were investigated for the first time in sp. The cultures harvested under 26 different conditions were analysed for pH, dissolved CO concentration, and the biomass of extracellular polysaccharides. The BX1.5 strain could thrive in a wide range of initial medium pH ranging from 3 to 11 and produced valuable lipids such as C16:0, C18:2, and C18:3 under indoor and outdoor culture conditions when supplied with 2.0% dissolved CO. Particularly, the acidic BG11 medium effectively increased the biomass of extracellular polysaccharides during short-term outdoor cultivation. The BG11 liquid medium also led to extracellular polysaccharide production, independent of acidity and alkalinity, proportional to the increase in total sugars derived from cells supplied with high CO concentrations. These results suggest as a promising strain for indoor and outdoor cultivation to produce valuable materials.

摘要

首次研究了在室内（50 - 450毫升规模）和室外（100 - 500升规模）培养能产生有用细胞内脂质和细胞外多糖的绿藻BX1.5菌株的高效pH值和溶解二氧化碳条件。对在26种不同条件下收获的培养物进行了pH值、溶解二氧化碳浓度和细胞外多糖生物量的分析。BX1.5菌株能在3至11的广泛初始培养基pH范围内生长，在室内和室外培养条件下，当提供2.0%的溶解二氧化碳时，能产生有价值的脂质，如C16:0、C18:2和C18:3。特别是，酸性BG11培养基在短期室外培养期间有效地增加了细胞外多糖的生物量。BG11液体培养基也导致细胞外多糖的产生，与酸碱度无关，与高浓度二氧化碳供应的细胞中总糖的增加成比例。这些结果表明该菌株是室内和室外培养生产有价值材料的有前途的菌株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/396db3bb4c65/fbioe-11-1233944-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/798a65a6730b/fbioe-11-1233944-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/2c9a71d97d4d/fbioe-11-1233944-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/23daf449258e/fbioe-11-1233944-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/f97d0c5089dd/fbioe-11-1233944-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/c3b16983b04b/fbioe-11-1233944-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/ee8320637d9c/fbioe-11-1233944-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/396db3bb4c65/fbioe-11-1233944-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/798a65a6730b/fbioe-11-1233944-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/2c9a71d97d4d/fbioe-11-1233944-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/23daf449258e/fbioe-11-1233944-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/f97d0c5089dd/fbioe-11-1233944-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/c3b16983b04b/fbioe-11-1233944-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/ee8320637d9c/fbioe-11-1233944-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc83/10520278/396db3bb4c65/fbioe-11-1233944-g007.jpg

相似文献

1
Efficient pH and dissolved CO conditions for indoor and outdoor cultures of green alga .绿藻室内和室外培养的有效pH值和溶解二氧化碳条件
Front Bioeng Biotechnol. 2023 Sep 11;11:1233944. doi: 10.3389/fbioe.2023.1233944. eCollection 2023.
2
Coproduction of lipids and extracellular polysaccharides from the novel green alga sp. BX1.5 depending on cultivation conditions.新型绿藻sp. BX1.5脂质与胞外多糖的联产及其对培养条件的依赖性
Biotechnol Rep (Amst). 2019 Nov 21;25:e00392. doi: 10.1016/j.btre.2019.e00392. eCollection 2020 Mar.
3
Characteristics and function of an extracellular polysaccharide from a green alga Parachlorella.一种绿藻 Parachlorella 胞外多糖的特性与功能。
Carbohydr Polym. 2021 Feb 15;254:117252. doi: 10.1016/j.carbpol.2020.117252. Epub 2020 Nov 2.
4
Screening of Isochrysis Strains and Utilization of a Two-Stage Outdoor Cultivation Strategy for Algal Biomass and Lipid Production.筛选小球藻菌株并利用两段式室外培养策略进行藻类生物量和脂质生产。
Appl Biochem Biotechnol. 2018 Aug;185(4):1100-1117. doi: 10.1007/s12010-018-2717-3. Epub 2018 Feb 17.
5
The microalga Parachlorella kessleri--a novel highly efficient lipid producer.小球藻——一种新型高效产脂微生物。
Biotechnol Bioeng. 2013 Jan;110(1):97-107. doi: 10.1002/bit.24595. Epub 2012 Jul 18.
6
Rescuing ethanol photosynthetic production of cyanobacteria in non-sterilized outdoor cultivations with a bicarbonate-based pH-rising strategy.采用基于碳酸氢盐的pH值升高策略，在未灭菌的室外培养中挽救蓝藻的乙醇光合生产。
Biotechnol Biofuels. 2017 Apr 14;10:93. doi: 10.1186/s13068-017-0765-5. eCollection 2017.
7
Supra-Optimal Temperature: An Efficient Approach for Overaccumulation of Starch in the Green Alga .超适温：一种在绿藻中过度积累淀粉的有效方法。
Cells. 2021 Jul 16;10(7):1806. doi: 10.3390/cells10071806.
8
Extracellular carotenoid production and fatty acids profile of Parachlorella kessleri under increased CO concentrations.高浓度 CO 下鞘藻属（Parachlorella kessleri）胞外类胡萝卜素的生产和脂肪酸组成。
J Biotechnol. 2021 Mar 10;329:151-159. doi: 10.1016/j.jbiotec.2021.02.004. Epub 2021 Feb 13.
9
Cultivation of Chlorella sp. using raw dairy wastewater for nutrient removal and biodiesel production: Characteristics comparison of indoor bench-scale and outdoor pilot-scale cultures.利用生乳废水培养小球藻去除营养物质和生产生物柴油：室内台架规模和室外中试规模培养的特性比较。
Bioresour Technol. 2015 Sep;192:382-8. doi: 10.1016/j.biortech.2015.05.094. Epub 2015 May 28.
10
Characterization of a novel marine unicellular alga, Pseudoneochloris sp. strain NKY372003 as a high carbohydrate producer.新型海洋单细胞藻类 Pseudoneochloris sp. 菌株 NKY372003 的特性研究及其作为高碳水化合物生产菌的潜力。
J Biosci Bioeng. 2020 Jun;129(6):687-692. doi: 10.1016/j.jbiosc.2019.12.010. Epub 2020 Jan 11.

引用本文的文献

1
Co-Culture of Cyanobacteria and Rhizobia, Increasing Biomass Under Nitrogen-Starvation Conditions.蓝细菌与根瘤菌的共培养，在氮饥饿条件下增加生物量。
Appl Biochem Biotechnol. 2025 Sep 1. doi: 10.1007/s12010-025-05353-7.
2
Characterization and Function of a Novel Extracellular Polysaccharide from a Green Alga Parachlorella sp. AMI5.一种来自绿藻Parachlorella sp. AMI5的新型细胞外多糖的表征与功能
Appl Biochem Biotechnol. 2025 Jun 11. doi: 10.1007/s12010-025-05276-3.
3
Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria.

本文引用的文献

1
Coproduction of lipids and carotenoids by the novel green alga sp. depending on cultivation conditions.新型绿藻根据培养条件共同产生脂质和类胡萝卜素。
Biotechnol Rep (Amst). 2022 Oct 24;37:e00769. doi: 10.1016/j.btre.2022.e00769. eCollection 2023 Mar.
2
Microalgae-Based Biorefineries: Challenges and Future Trends to Produce Carbohydrate Enriched Biomass, High-Added Value Products and Bioactive Compounds.基于微藻的生物精炼厂：生产富含碳水化合物的生物质、高附加值产品和生物活性化合物的挑战与未来趋势。
Biology (Basel). 2022 Jul 29;11(8):1146. doi: 10.3390/biology11081146.
3
Characteristics and function of an extracellular polysaccharide from a green alga Parachlorella.
烷烃生物合成基因表达及其在重组蓝细菌中的产量增加。
FEBS Open Bio. 2025 Jun;15(6):949-962. doi: 10.1002/2211-5463.70009. Epub 2025 Mar 6.
一种绿藻 Parachlorella 胞外多糖的特性与功能。
Carbohydr Polym. 2021 Feb 15;254:117252. doi: 10.1016/j.carbpol.2020.117252. Epub 2020 Nov 2.
4
Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.微藻的生物工程：工业应用的最新进展、前景及监管挑战
Front Bioeng Biotechnol. 2020 Sep 3;8:914. doi: 10.3389/fbioe.2020.00914. eCollection 2020.
5
Coproduction of lipids and extracellular polysaccharides from the novel green alga sp. BX1.5 depending on cultivation conditions.新型绿藻sp. BX1.5脂质与胞外多糖的联产及其对培养条件的依赖性
Biotechnol Rep (Amst). 2019 Nov 21;25:e00392. doi: 10.1016/j.btre.2019.e00392. eCollection 2020 Mar.
6
The promising future of microalgae: current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products.微藻的光明前景：生物燃料、饲料和其他产品的可持续可再生产业的现状、挑战和优化。
Microb Cell Fact. 2018 Mar 5;17(1):36. doi: 10.1186/s12934-018-0879-x.
7
Kinetics of growth and lipids accumulation in Chlorella vulgaris during batch heterotrophic cultivation: Effect of different nutrient limitation strategies.小球藻分批异养培养过程中的生长和油脂积累动力学：不同营养限制策略的影响。
Bioresour Technol. 2017 Nov;243:356-365. doi: 10.1016/j.biortech.2017.06.110. Epub 2017 Jun 23.
8
Effect of pH on growth and lipid accumulation kinetics of the microalga Chlorella vulgaris grown heterotrophically under sulfur limitation.在硫限制条件下异养生长的小球藻生长和脂类积累动力学的 pH 值影响。
Bioresour Technol. 2016 Nov;219:694-701. doi: 10.1016/j.biortech.2016.08.033. Epub 2016 Aug 12.
9
Spirulan from blue-green algae inhibits fibrin and blood clots: its potent antithrombotic effects.蓝藻中的螺旋藻抑制纤维蛋白和血栓形成：其强大的抗血栓作用。
J Biochem Mol Toxicol. 2015 May;29(5):240-8. doi: 10.1002/jbt.21690. Epub 2015 Feb 4.
10
Air-drying of cells, the novel conditions for stimulated synthesis of triacylglycerol in a Green Alga, Chlorella kessleri.细胞的风干，小球藻中三酰基甘油受刺激合成的新条件。
PLoS One. 2013 Nov 15;8(11):e79630. doi: 10.1371/journal.pone.0079630. eCollection 2013.