Jiang Liying, Lan Xianghao, Ren Linjiao, Jin Zhiyuan, Shan Xuchen, Yang Mingzhu, Chang Lingqian
School of Electrical and Information Engineering, Zhengzhou University of Light Industry, 450002 Zhengzhou, China.
Academy for Quantum Science and Technology, Zhengzhou University of Light Industry, 450002 Zhengzhou, China.
Microsyst Nanoeng. 2023 Sep 25;9:118. doi: 10.1038/s41378-023-00576-2. eCollection 2023.
To minimize and control the transmission of infectious diseases, a sensitive, accurate, rapid, and robust assay strategy for application on-site screening is critical. Here, we report single-molecule RNA capture-assisted digital RT-LAMP (SCADL) for point-of-care testing of infectious diseases. Target RNA was captured and enriched by specific capture probes and oligonucleotide probes conjugated to magnetic beads, replacing laborious RNA extraction. Droplet generation, amplification, and the recording of results are all integrated on a microfluidic chip. In assaying commercial standard samples, quantitative results precisely corresponded to the actual concentration of samples. This method provides a limit of detection of 10 copies mL for the N gene within 1 h, greatly reducing the need for skilled personnel and precision instruments. The ultrasensitivity, specificity, portability, rapidity and user-friendliness make SCADL a competitive candidate for the on-site screening of infectious diseases.
为了最小化和控制传染病的传播，一种用于现场筛查的灵敏、准确、快速且稳健的检测策略至关重要。在此，我们报告了用于传染病即时检测的单分子RNA捕获辅助数字逆转录环介导等温扩增技术（SCADL）。通过与磁珠偶联的特异性捕获探针和寡核苷酸探针捕获并富集目标RNA，取代了繁琐的RNA提取过程。液滴生成、扩增以及结果记录均集成在微流控芯片上。在检测商业标准样品时，定量结果与样品的实际浓度精确对应。该方法对N基因的检测限为每毫升10个拷贝，1小时内即可完成检测，极大减少了对技术人员和精密仪器的需求。其超灵敏度、特异性、便携性、快速性和用户友好性使SCADL成为传染病现场筛查的有力候选方法。
