Department of Gastroenterology, The First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou, China.
Clinical Laboratory, The First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou, China.
Genet Test Mol Biomarkers. 2023 Sep;27(9):284-289. doi: 10.1089/gtmb.2023.0107.
Apolipoprotein A5 (APOA5) is involved in serum triglyceride (TG) regulation. Several studies have reported that the rs651821 locus in the gene is associated with serum TG levels in the Chinese population. However, no research has been performed regarding the association between the variants of rs651821 and the risk of hyperlipidemic acute pancreatitis (HLAP). A case-control study was conducted and is reported following the STROBE guidelines. We enrolled a total of 88 participants in this study (60 HLAP patients and 28 controls). was genotyped using PCR and Sanger sequencing. Logistic regression models were conducted to calculate odds ratios and a 95% confidence interval. The genotype distribution of the rs651821 alleles in both groups follow the Hardy-Weinberg distribution. The frequency of the "C" allele in rs651821 was increased in HLAP patients compared to controls. In the recessive model, subjects with the "CC" genotype had an 8.217-fold higher risk for HLAP (OR = 8.217, 95% CI: 1.023-66.01, = 0.046) than subjects with the "TC+TT" genotypes. After adjusting for sex, the association remained significant (OR = 9.898, 95% CI: 1.176-83.344, = 0.035). Additionally, the "CC" genotype was related to an increased TG/apolipoprotein B (APOB) ratio and fasting plasma glucose (FPG) levels. Our findings suggest that the C allele of rs651821 in increases the risk of HLAP in persons from Southeastern China.
载脂蛋白 A5 (APOA5) 参与血清甘油三酯 (TG) 的调节。多项研究报道，基因中的 rs651821 位点与中国人群的血清 TG 水平相关。然而，尚未研究 rs651821 变异与高脂血症性急性胰腺炎 (HLAP) 风险之间的关系。本研究采用病例对照研究，并按照 STROBE 指南进行报告。我们共纳入了 88 名参与者（60 名 HLAP 患者和 28 名对照）。使用 PCR 和 Sanger 测序进行基因型分型。采用 logistic 回归模型计算比值比和 95%置信区间。两组 rs651821 等位基因的基因型分布均符合 Hardy-Weinberg 分布。与对照组相比，HLAP 患者 rs651821 的 "C" 等位基因频率增加。在隐性模型中，与 "TC+TT" 基因型相比，"CC" 基因型的个体发生 HLAP 的风险增加 8.217 倍（OR=8.217，95%CI：1.023-66.01， =0.046）。在校正性别后，关联仍然显著（OR=9.898，95%CI：1.176-83.344， =0.035）。此外，"CC" 基因型与 TG/载脂蛋白 B (APOB) 比值和空腹血糖 (FPG) 水平升高有关。我们的研究结果表明，载脂蛋白 A5 基因中的 rs651821 等位基因 C 增加了中国东南部人群 HLAP 的发病风险。
