Arivarasan Vishnu Kirthi
Department of Microbiology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India.
Prog Mol Biol Transl Sci. 2023;201:133-149. doi: 10.1016/bs.pmbts.2023.03.025. Epub 2023 Apr 20.
Phages, viruses that infect bacteria, have been explored as promising tools for the detection of human disease. By leveraging the specificity of phages for their bacterial hosts, phage-based diagnostic tools can rapidly and accurately detect bacterial infections in clinical samples. In recent years, advances in genetic engineering and biotechnology have enabled the development of more sophisticated phage-based diagnostic tools, including those that express reporter genes or enzymes, or target specific virulence factors or antibiotic resistance genes. However, despite these advancements, there are still challenges and limitations to the use of phage-based diagnostic tools, including concerns over phage safety and efficacy. This review aims to provide a comprehensive overview of the current state of phage-based diagnostic tools, including their advantages, limitations, and potential for future development. By addressing these issues, we hope to contribute to the ongoing efforts to develop safe and effective phage-based diagnostic tools for the detection of human disease.
噬菌体，即感染细菌的病毒，已被探索作为检测人类疾病的有前景的工具。通过利用噬菌体对其细菌宿主的特异性，基于噬菌体的诊断工具可以快速、准确地检测临床样本中的细菌感染。近年来，基因工程和生物技术的进展使得开发更复杂的基于噬菌体的诊断工具成为可能，包括那些表达报告基因或酶、或靶向特定毒力因子或抗生素抗性基因的工具。然而，尽管有这些进展，基于噬菌体的诊断工具的使用仍然存在挑战和局限性，包括对噬菌体安全性和有效性的担忧。本综述旨在全面概述基于噬菌体的诊断工具的现状，包括其优点、局限性以及未来发展潜力。通过解决这些问题，我们希望为正在进行的开发用于检测人类疾病的安全有效的基于噬菌体的诊断工具的努力做出贡献。
