Department of Surgery, Asahikawa Medical University, Midorigaoka higashi 2-1-1, Asahikawa, Hokkaido 078-8510, Japan; Department of Development of Transplantation Technology, Asahikawa Medical University, Midorigaoka higashi 2-1-1, Asahikawa, Hokkaido 078-8510, Japan.
Department of Surgery, Asahikawa Medical University, Midorigaoka higashi 2-1-1, Asahikawa, Hokkaido 078-8510, Japan; Department of Development of Transplantation Technology, Asahikawa Medical University, Midorigaoka higashi 2-1-1, Asahikawa, Hokkaido 078-8510, Japan.
Transplant Proc. 2023 Nov;55(9):2212-2217. doi: 10.1016/j.transproceed.2023.07.031. Epub 2023 Sep 26.
The University of Wisconsin (UW) solution is the gold standard for preserving the liver, kidneys, and pancreas. For renal preservation, the addition of the flavonoid, quercetin (QE), to the preservation solution reduces damage to renal tubular cells, and the addition of sucrose (Suc) is also beneficial for preservation. The aim of this study was to investigate the protective effects of QE and Suc on porcine livers in terms of warm and cold injury and to evaluate whether their use improves ischemia-reperfusion (I/R) injury after simple cold storage (CS).
We tested porcine livers procured after 30 minutes of warm ischemia followed by preservation for 6 hours under the following 2 conditions: group 1, preserved with the CS/UW solution (n = 4); group 2, preserved with the CS/UW solution containing Que 33.1 μM and Suc 0.1 M (n = 6). All livers were evaluated using an ex vivo isolated liver reperfusion model with saline-diluted autologous blood.
Aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase levels in group 2 were significantly lower at 30 minutes of reperfusion than in group 1. Furthermore, histologic evaluation by hematoxylin and eosin staining showed significantly fewer morphologic changes in group 2 than in group 1, as indicated by the total Suzuki score. Group 2 also had significantly better scores for sinusoidal congestion and hepatocyte cytoplasmic vacuolization.
Adding Que and Suc to the UW solution can effectively prevent cold injury in livers donated after circulatory death.
威斯康星大学（UW）溶液是保存肝脏、肾脏和胰腺的金标准。对于肾脏保存，在保存溶液中添加类黄酮槲皮素（QE）可减少对肾小管细胞的损伤，添加蔗糖（Suc）也有利于保存。本研究旨在探讨 QE 和 Suc 对猪肝脏在温热和冷损伤方面的保护作用，并评估它们在单纯低温保存（CS）后是否改善缺血再灌注（I/R）损伤。
我们在以下两种情况下测试了经过 30 分钟热缺血后获得的猪肝脏，并在 6 小时内进行保存：组 1，用 CS/UW 溶液保存（n=4）；组 2，用含有 33.1 μM QE 和 0.1 M Suc 的 CS/UW 溶液保存（n=6）。所有肝脏均使用含有生理盐水稀释的自体血液的离体肝脏再灌注模型进行评估。
在再灌注 30 分钟时，组 2 的天冬氨酸转氨酶、丙氨酸转氨酶和乳酸脱氢酶水平明显低于组 1。此外，通过苏木精和伊红染色的组织学评估显示，组 2 的形态学变化明显少于组 1，总 Suzuki 评分表明。组 2 的窦状隙充血和肝细胞细胞质空泡化的评分也明显更好。
在 UW 溶液中添加 Que 和 Suc 可以有效预防循环死亡后捐献的肝脏的冷损伤。
