Molina-Menor Esther, Vidal-Verdú Àngela, Gomis-Olcina Carlos, Peretó Juli, Porcar Manuel
Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, Spain.
Departament de Bioquímica i Biologia Molecular, Universitat de València, Burjassot, Spain.
Front Bioeng Biotechnol. 2023 Sep 12;11:1250667. doi: 10.3389/fbioe.2023.1250667. eCollection 2023.
Solid State Fermentation (SSF) processes have been explored for yeast growth and protein and metabolites production. However, most of these processes lack standardization. In this work, we present a polylactic acid (PLA) 3D printed matrix that dramatically enhances yeast growth when embedded in liquid media compared to equivalent static cultures, and changes yeast expression patterns at the proteome level (data are available via ProteomeXchange with identifier PXD043759). Moreover, differences in sugar assimilation and ethanol production, as the main product of alcoholic fermentation, are observed. Our results suggest that these matrixes may be useful for a vast range of biotechnological applications based on yeast fermentation.
固态发酵（SSF）工艺已被用于酵母生长以及蛋白质和代谢产物的生产研究。然而，这些工艺大多缺乏标准化。在本研究中，我们展示了一种聚乳酸（PLA）3D打印基质，与同等的静态培养相比，当将其嵌入液体培养基时，能显著促进酵母生长，并在蛋白质组水平改变酵母的表达模式（数据可通过ProteomeXchange获取，标识符为PXD043759）。此外，还观察到了酵母在糖同化和乙醇生产（作为酒精发酵的主要产物）方面的差异。我们的结果表明，这些基质可能对基于酵母发酵的广泛生物技术应用有用。
