Song Weidu, Zhang Chuanhao, Lin Huichao, Zhang Taiyi, Liu Haixia, Huang Xiaowen
State Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, China.
Lab Chip. 2023 Oct 10;23(20):4592-4599. doi: 10.1039/d3lc00401e.
The rapid quantitative detection of in milk is of great significance to food safety. Quantitative real-time polymerase chain reaction (qPCR) technology is a good choice to meet this requirement. A good qPCR system should show the advantages of being low cost, having low-power consumption, having potential for miniaturization and be portable. However, most of the time-domain-based qPCR systems reported to date do not meet these requirements. In this study, we propose a novel real-time rotary PCR reaction system (RRP) that meets all the abovementioned specifications, and contains four modules: a heating control module, a disposable PCR capillary tube, a mechanical control module, and a photoelectric detection module. The volume of our homemade-PCR capillary tube is only 3 μL. The total manufacturing cost is cheaper than $200, and the capillary tube is about 1.4 cents. The size parameter of the RRP is less than 300 mm × 150 mm × 150 mm, using low mobile power sources to operate. All the features mean that the RRP meets the advantages of low sample volumes, enhanced thermal conductivity and being portable. Through conducting the experimental quantitative detection of in milk and theoretical simulations by COMSOL, we prove the feasibility of this rotary PCR real-time detection system, which has broad application prospects in the rapid detection of bacteria and food safety.
牛奶中[具体物质未给出]的快速定量检测对食品安全具有重要意义。定量实时聚合酶链反应（qPCR）技术是满足这一要求的良好选择。一个良好的qPCR系统应具备低成本、低功耗、具有小型化潜力且便于携带的优点。然而，迄今为止报道的大多数基于时域的qPCR系统都不符合这些要求。在本研究中，我们提出了一种新颖的实时旋转PCR反应系统（RRP），它满足上述所有规格，并且包含四个模块：一个加热控制模块、一个一次性PCR毛细管、一个机械控制模块和一个光电检测模块。我们自制的PCR毛细管体积仅为3 μL。总制造成本低于200美元，毛细管约为1.4美分。RRP的尺寸参数小于300 mm×150 mm×150 mm，使用低移动电源运行。所有这些特点意味着RRP具备样品体积小、热导率增强和便于携带的优点。通过对牛奶中[具体物质未给出]进行实验定量检测以及利用COMSOL进行理论模拟，我们证明了这种旋转PCR实时检测系统的可行性，其在细菌快速检测和食品安全方面具有广阔的应用前景。
