Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
School of Medicine, Walailak University, Nakhon Si Thammarat, Thailand.
Anticancer Res. 2023 Oct;43(10):4461-4472. doi: 10.21873/anticanres.16641.
BACKGROUND/AIM: Colorectal cancer (CRC) is strongly associated with altered cadherin adhesion molecules. Oxaliplatin is a standard treatment for CRC, yet high-doses have concerning side effects. In this study, the effects of oxaliplatin and the combination of oxaliplatin with vitamin C on HCT-116 CRC cell migration and invasion were studied through the roles of cellular oxidative stress associated with cadherin molecules.
The cellular assays used in this research were MTT, DCFH-DA, immunofluorescence, and western blotting. Cancer progression was examined using wound healing and Boyden chamber techniques.
The results indicate that hydrogen peroxide-induced cellular oxidative stress induced cancer cell migration and invasion. The combined treatment of oxaliplatin with a pro-oxidant concentration of vitamin C resulted in higher toxicity than treatment with oxaliplatin alone. However, treatment with the combination of oxaliplatin and antioxidant concentrations of vitamin C suppressed cancer migration and invasion. Furthermore, the combination treatment increased E-cadherin expression, whereas decreased that of N-cadherin.
Treatment with the combination of oxaliplatin with vitamin C can inhibit CRC cell growth and decrease cancer cell migration and invasion, via oxidative stress and cadherins.
背景/目的：结直肠癌（CRC）与细胞黏附分子中 cadherin 的改变密切相关。奥沙利铂是 CRC 的标准治疗方法，但高剂量有令人担忧的副作用。在这项研究中，通过与 cadherin 分子相关的细胞氧化应激作用，研究了奥沙利铂和奥沙利铂与维生素 C 联合治疗对 HCT-116 CRC 细胞迁移和侵袭的影响。
本研究中使用的细胞检测方法包括 MTT、DCFH-DA、免疫荧光和 Western blot。使用划痕愈合和 Boyden 室技术检测癌症进展。
结果表明，过氧化氢诱导的细胞氧化应激诱导癌细胞迁移和侵袭。奥沙利铂与促氧化剂浓度的维生素 C 联合治疗比单独使用奥沙利铂的毒性更高。然而，奥沙利铂与抗氧化剂浓度的维生素 C 的联合治疗抑制了癌症的迁移和侵袭。此外，联合治疗增加了 E-cadherin 的表达，而降低了 N-cadherin 的表达。
奥沙利铂与维生素 C 的联合治疗可通过氧化应激和 cadherin 抑制 CRC 细胞生长并降低癌细胞迁移和侵袭。
