Department of Biomedical Science, Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima, Japan
Department of Biomedical Science, Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima, Japan.
Anticancer Res. 2023 Oct;43(10):4327-4331. doi: 10.21873/anticanres.16627.
Neuroblastoma is the most common extracranial solid tumor in children, and the overall survival rate of patients in the high-risk group is less than 50%. Circulating tumor cells have recently been found to be a potential source of tumor progression, metastasis, and poor prognosis in patients with cancer. There is a dearth of data on the malignant potential of circulating tumor cells in neuroblastoma. Therefore, in this article, we briefly review the methods of quantifying circulating neuroblastoma cells and the relationship between the presence of many circulating tumor cells and prognosis for patients with neuroblastoma. The relationship between higher numbers of circulating neuroblastoma tumor cells and poor prognosis was outlined. However, the data in most studies represented only the tumor burden, and there is no direct evidence that circulating tumor cells are the source of tumor progression and metastasis in patients with neuroblastoma. A comprehensive genetic analysis of circulating tumor cells, including single-cell sequencing and functional assays using ex vivo cell culture and xenografts, may provide insights into the malignant nature of neuroblastoma.
神经母细胞瘤是儿童最常见的颅外实体瘤，高危组患者的总体生存率低于 50%。最近发现，循环肿瘤细胞是癌症患者肿瘤进展、转移和预后不良的潜在来源。关于神经母细胞瘤循环肿瘤细胞的恶性潜能的数据很少。因此，在本文中，我们简要回顾了定量检测循环神经母细胞瘤细胞的方法，以及循环肿瘤细胞数量与神经母细胞瘤患者预后之间的关系。概述了更多循环神经母细胞瘤肿瘤细胞数量与不良预后之间的关系。然而，大多数研究中的数据仅代表肿瘤负担，没有直接证据表明循环肿瘤细胞是神经母细胞瘤患者肿瘤进展和转移的来源。对循环肿瘤细胞进行全面的遗传分析，包括使用体外细胞培养和异种移植进行单细胞测序和功能检测，可能有助于了解神经母细胞瘤的恶性本质。
