Inducible Cytochrome P450s in the Fat Body and Malpighian Tubules of the Polyphagous Pests of Confer Xenobiotic Tolerance.

Cytochrome P450 plays vital roles in detoxifying xenobiotics. In this study, and expression in the fat body and , , , and expression in the Malpighian tubules were significantly upregulated after cyantraniliprole exposure, and and expression levels were simultaneously increased in the Malpighian tubules after gossypol treatment. ectopically expressing candidate P450 genes showed that , , , and conferred cyantraniliprole tolerance. The overexpression of and in increased the number of eggs laid under the gossypol treatment. Moreover, the knockdown of , , , and increased mortality under the cyantraniliprole treatment. Homology modeling and molecular docking results suggested that candidate P450 has the potential to bind with cyantraniliprole. These results indicate that the CYP3 and CYP4 genes participate in cyantraniliprole detoxification and that may be simultaneously involved in the gossypol tolerance of .