生酮细菌发酵产生的2-酮基-L-古洛糖酸及其他酮醛糖酸和醛糖酸的测定。

Determination of 2-keto-L-gulonic and other ketoaldonic and aldonic acids produced by ketogenic bacterial fermentation.

作者信息

Lazarus R A, Seymour J L

出版信息

Anal Biochem. 1986 Sep;157(2):360-6. doi: 10.1016/0003-2697(86)90638-x.

Abstract

The quantitative analysis of 2-keto-L-gulonic acid (2-KLG) produced by microbial fermentation is described. 2-KLG is separated from other aldonic and ketoaldonic acids by high-performance liquid chromatography on an Aminex anion exchange column with ammonium formate or potassium phosphate as the eluant. This is a rapid and simple method for routine analysis of a large number of samples generated by fermentation studies. Gas chromatography--mass spectrometry permits the qualitative and quantitative analysis of nanogram levels of 2-keto-L-gulonate in complex media and provides confirmation of the HPLC results. The methodologies presented are useful for the analysis of a number of aldonic and ketoaldonic acids.

摘要

本文描述了对微生物发酵产生的2-酮基-L-古洛糖酸（2-KLG）的定量分析方法。2-KLG通过高效液相色谱法在Aminex阴离子交换柱上，以甲酸铵或磷酸钾作为洗脱剂，与其他醛糖酸和酮醛糖酸分离。这是一种快速且简单的方法，适用于对发酵研究中产生的大量样品进行常规分析。气相色谱-质谱联用技术可对复杂培养基中纳克级的2-酮基-L-古洛糖酸盐进行定性和定量分析，并能对高效液相色谱的结果进行确认。所介绍的方法对于多种醛糖酸和酮醛糖酸的分析都很有用。