Xiang Shi, Li Jingxian, Wang Futing, Yang Hongfen, Jiang Yifei, Zhang Penghui, Cai Ren, Tan Weihong
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, Hunan 410082, China.
Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.
Anal Chem. 2023 Oct 10;95(40):15125-15132. doi: 10.1021/acs.analchem.3c03437. Epub 2023 Sep 29.
An ultralow-potential electrochemiluminescence (ECL) aptasensor has been designed for zearalenone (ZEN) assay based on a resonance energy transfer (RET) system with SnS QDs/g-CN as a novel luminophore and CuO/NH-UiO-66 as a dual-quencher. SnS QDs were loaded onto g-CN nanosheets and enhanced the ECL luminescence via strong synergistic effects under an ultralow potential. The UV-vis absorption spectrum of CuO/NH-UiO-66 exhibits considerable overlap with the ECL emission spectrum of SnS QDs/g-CN, an important consideration for the RET process. In order to stimulate RET, the ZEN aptamer and complementary DNA are introduced for conjugation between the donor and the acceptor. With the binding interaction between ZEN by its aptamer, CuO/NH-UiO-66 is removed from the electrode surface, resulting in the inhibition of the RET system and an increase in the ECL signal. Under optimal conditions, the as-prepared aptasensor quantified ZEN from 0.5 μg·mL to 0.1 fg·mL with a low limit of detection of 0.085 fg·mL, and it exhibited good stability, excellent specificity, high reproducibility, and desirable practicality. The sensing strategy provides a method for mycotoxins assay to monitor food safety.
基于以SnS量子点/g-CN作为新型发光体、CuO/NH-UiO-66作为双猝灭剂的共振能量转移（RET）系统，设计了一种用于检测玉米赤霉烯酮（ZEN）的超低电位电化学发光（ECL）适配体传感器。将SnS量子点负载到g-CN纳米片上，并在超低电位下通过强协同效应增强ECL发光。CuO/NH-UiO-66的紫外-可见吸收光谱与SnS量子点/g-CN的ECL发射光谱有相当大的重叠，这是RET过程的一个重要考虑因素。为了激发RET，引入ZEN适配体和互补DNA用于供体和受体之间的共轭。随着ZEN与其适配体的结合相互作用，CuO/NH-UiO-66从电极表面被去除，导致RET系统受到抑制，ECL信号增强。在最佳条件下，所制备的适配体传感器对ZEN的定量检测范围为0.5 μg·mL至0.1 fg·mL，检测限低至0.085 fg·mL，并且表现出良好的稳定性、优异的特异性、高重现性和理想的实用性。该传感策略为监测食品安全的霉菌毒素检测提供了一种方法。
