Department of Pharmacy, Anning First People's Hospital Affiliated to Kunming University of Science and Technology, Anning, Yunnan 650302, China.
Department of Formulation Engineering, Henan Technician College of Medicine and Health, Kaifeng, Henan 475000, China.
J Reprod Immunol. 2023 Dec;160:104154. doi: 10.1016/j.jri.2023.104154. Epub 2023 Sep 20.
Pelvic inflammatory disease (PID) is commonly encountered in gynecological practice. Kangfuxiaomi suppository, made from the compound extract of Periplaneta Americana, is a Traditional Chinese Medicine remedy widely used for the treatment of gynecological disorders. This study aimed to preliminarily explore the therapeutic effect of Kangfuxiaomi suppository in a rat model of PID established by chemical injury and pathogen infection. The key parameters assessed were vulvar inflammation score, vaginal + uterine organ index, and serum levels of interleukin (IL)- 8; tumor necrosis factor (TNF)-α; C-reactive protein (CRP); superoxide dismutase (SOD); and malondialdehyde (MDA). In addition, levels of IL-6, cyclooxygenase (COX)- 2, and IL-2 in cervical tissues as well as that of IL-1β and prostaglandin E-2 (PGE2) in uterine tissues were measured. The expression levels of nuclear factor-kappa B (NF-κB) p65 and Toll-like receptor 4 (TLR4) in uterine tissues were detected by immunohistochemical method. After Kangfuxiaomi suppository treatment, the vulva inflammation score and histopathological score of PID rats showed a tendency to decrease. Serum IL-8, TNF-α, CRP, and MDA levels were reduced, while SOD levels were significantly increased. Levels of IL-6, IL-2, and COX-2 in cervical tissues were somewhat decreased, and PGE2 and IL-1β levels in uterine tissue were significantly decreased. Moreover, the levels of NF-κB p65 and TLR4 protein expression were also decreased. These findings demonstrated the therapeutic effect of Kangfuxiaomi suppository in PID rats. The underlying mechanism may involve enhanced antioxidant capacity and decreased secretion of proinflammatory factors via the NF-κB/TLR4 signaling pathway.
盆腔炎（PID）是妇科临床常见疾病。康复消炎栓是由美洲大蠊复方提取制成的中药制剂，广泛用于妇科疾病的治疗。本研究旨在初步探讨康复消炎栓对化学损伤和病原体感染致 PID 大鼠模型的治疗作用，评估指标包括外阴炎症评分、阴道+子宫脏器指数、血清白细胞介素（IL）-8、肿瘤坏死因子（TNF）-α、C 反应蛋白（CRP）、超氧化物歧化酶（SOD）、丙二醛（MDA）；宫颈组织 IL-6、环氧化酶（COX）-2、IL-2，以及子宫组织 IL-1β、前列腺素 E2（PGE2）的水平；免疫组化法检测子宫组织核因子-κB（NF-κB）p65、Toll 样受体 4（TLR4）的表达水平。经康复消炎栓治疗后，PID 大鼠外阴炎症评分和组织病理学评分均有下降趋势，血清 IL-8、TNF-α、CRP、MDA 水平降低，SOD 水平显著升高；宫颈组织 IL-6、IL-2、COX-2 水平有所降低，子宫组织 PGE2、IL-1β水平显著降低，NF-κB p65、TLR4 蛋白表达水平也降低。以上结果提示康复消炎栓对 PID 大鼠有治疗作用，其作用机制可能与增强机体抗氧化能力、通过 NF-κB/TLR4 信号通路减少促炎因子分泌有关。
