Department of Chemical and Materials Engineering, University of Nevada, Reno, Reno, Nevada, USA.
Department of Computer Science and Software Engineering, Auburn University, Auburn, Alabama, USA.
Proteins. 2024 Feb;92(2):157-169. doi: 10.1002/prot.26599. Epub 2023 Sep 30.
Acyltransferases (AT) are enzymes that catalyze the transfer of acyl group to a receptor molecule. This review focuses on ATs that act on thioester-containing substrates. Although many ATs can recognize a wide variety of substrates, sequence similarity analysis allowed us to classify the ATs into fifteen distinct families. Each AT family is originated from enzymes experimentally characterized to have AT activity, classified according to sequence similarity, and confirmed with tertiary structure similarity for families that have crystallized structures available. All the sequences and structures of the AT families described here are present in the thioester-active enzyme (ThYme) database. The AT sequences and structures classified into families and available in the ThYme database could contribute to enlightening the understanding acyl transfer to thioester-containing substrates, most commonly coenzyme A, which occur in multiple metabolic pathways, mostly with fatty acids.
酰基转移酶（AT）是催化酰基向受体分子转移的酶。本综述重点介绍作用于含硫酯底物的 AT。尽管许多 AT 可以识别多种底物，但序列相似性分析使我们能够将 AT 分为十五个不同的家族。每个 AT 家族都源自具有 AT 活性的实验表征的酶，根据序列相似性进行分类，并对已获得晶体结构的家族进行三级结构相似性确认。这里描述的所有 AT 家族的序列和结构都存在于硫酯活性酶（ThYme）数据库中。分类为家族并可在 ThYme 数据库中获得的 AT 序列和结构有助于阐明酰基向含硫酯底物的转移，这些底物通常是辅酶 A，存在于多种代谢途径中，大多数与脂肪酸有关。
