Faculty of Veterinary Medicine, Department of Internal Medicine, Selcuk University, Konya, Turkey.
Faculty of Veterinary Medicine, Department of Internal Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
Vet Med Sci. 2023 Nov;9(6):2893-2900. doi: 10.1002/vms3.1292. Epub 2023 Sep 30.
Displaced abomasum (DA) is one of the most important metabolic disorders of dairy cattle. In DA, ischaemic damage may occur as a result of impaired perfusion due to abomasal displacement, which may result in gastrointestinal mucosal damage.
Investigation of gastrointestinal tissue damage in cattle with right displacement of the abomasum (RDA) and left displacement of the abomasum (LDA) using intestinal-related biomarkers.
Forty-eight DA (24 LDA, 24 RDA) and 15 healthy Holstein dairy cows were enrolled between March 2021 and July 2022. Serum biomarkers including gamma-enteric smooth muscle actin (ACTG-2), liver-fatty acid binding proteins (L-FABP), platelet activating factor (PAF), trefoil factor-3 (TFF-3), leptin, claudin-3 and interleukin-8 (IL-8) concentrations were measured from venous blood samples.
L-FABP concentrations in the LDA group and TFF-3 concentrations in the RDA group were lower than in the control group. The leptin concentration of the RDA group was higher than that of the other groups. There was a negative correlation between lactate, leptin and IL-8 concentrations. There was a negative correlation between lactate and TFF-3, whereas leptin and lactate were positively correlated. Leptin was the more reliable biomarker for discriminating between RDA and LDA cases.
Changes in serum L-FABP, TFF-3 and leptin concentrations in cattle with DA may reflect acute intestinal injury and the subsequent repair phase. However, these biomarkers had poor diagnostic performance in discriminating between healthy and cattle with DA, while leptin emerged as the most useful marker in differentiating LDA from RDA cases.
皱胃移位（DA）是奶牛最重要的代谢紊乱之一。在 DA 中，由于皱胃移位导致灌注受损可能会发生缺血性损伤，从而导致胃肠道黏膜损伤。
使用与胃肠道相关的生物标志物研究患有右方变位性皱胃炎（RDA）和左方变位性皱胃炎（LDA）的奶牛的胃肠道组织损伤。
2021 年 3 月至 2022 年 7 月期间，共纳入 48 例 DA（24 例 LDA，24 例 RDA）和 15 例健康荷斯坦奶牛。从静脉血样中测量了包括γ-肠平滑肌肌动蛋白（ACTG-2）、肝脂肪酸结合蛋白（L-FABP）、血小板激活因子（PAF）、三叶因子-3（TFF-3）、瘦素、闭合蛋白-3 和白细胞介素-8（IL-8）在内的血清生物标志物浓度。
LDA 组的 L-FABP 浓度和 RDA 组的 TFF-3 浓度低于对照组。RDA 组的瘦素浓度高于其他组。乳酸、瘦素和 IL-8 浓度之间呈负相关。乳酸与 TFF-3 呈负相关，而瘦素与乳酸呈正相关。瘦素是区分 RDA 和 LDA 病例的更可靠的生物标志物。
患有 DA 的奶牛血清中 L-FABP、TFF-3 和瘦素浓度的变化可能反映了急性肠损伤及其随后的修复阶段。然而，这些生物标志物在区分健康牛和患有 DA 的牛方面的诊断性能较差，而瘦素在区分 LDA 和 RDA 病例方面表现最为有用。
