Department of Anatomy, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Republic of Korea.
Department of Anatomy, Yonsei University College of Medicine, Seoul, Republic of Korea.
Methods Mol Biol. 2024;2711:13-20. doi: 10.1007/978-1-0716-3429-5_2.
Inflammation in vascular structures due to external factors such as injury or infection inevitably leads to blood leakage. Therefore, measuring blood infiltrated into tissue may serve as an indication for the extent of an inflammatory reaction or injury. There are various methods of confirming vascular permeability in vivo and in vitro; for example, using a blood vessel permeable dye, the dye efflux can be quantitatively measured with a spectrophotometer. Although the aforementioned commonly used methods can measure leaked dye without difficulty, substantial limitations exist regarding the time points of blood leakage that can be measured. Here, we describe the details of a novel protocol to identify and analyze the real-time progression of blood leakage in vivo. This method, by combining existing methods with real-time imaging, is expected to immensely improve the visualization and evaluation of vascular permeability.
由于外伤或感染等外部因素导致的血管结构炎症不可避免地会导致血液渗漏。因此，测量血液渗入组织的程度可以作为炎症反应或损伤程度的指标。有多种方法可以在体内和体外确认血管通透性；例如，使用血管通透性染料，可以用分光光度计定量测量染料的流出量。尽管上述常用方法可以毫不费力地测量泄漏的染料，但在可以测量的血液泄漏时间点方面存在很大的局限性。在这里，我们描述了一种新的方案，用于识别和分析体内实时的血液泄漏进展。这种方法通过将现有的方法与实时成像相结合，有望极大地提高血管通透性的可视化和评估。
