School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi 'an 710021, China.
School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi 'an 710021, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 6):127141. doi: 10.1016/j.ijbiomac.2023.127141. Epub 2023 Sep 28.
Kiwifruit pomace is abundant in polysaccharides that exhibit diverse biological activities and prebiotic potential. This study delves into the digestive behavior and fermentation characteristics of kiwifruit pomace polysaccharides (KFP) through an in vitro simulated saliva-gastrointestinal digestion and fecal fermentation. The results reveal that following simulated digestion of KFP, its molecular weight reduced by 4.7%, and the reducing sugar (C) increased by 9.5%. However, the monosaccharide composition and Fourier transform infrared spectroscopy characteristics showed no significant changes, suggesting that KFP remained undigested. Furthermore, even after saliva-gastrointestinal digestion, KFP retained in vitro hypolipidemic and hypoglycemic activities. Subsequently, fecal fermentation significantly altered the physicochemical properties of indigestible KFP (KFPI), particularly leading to an 89.71% reduction in C. This indicates that gut microbiota could decompose KFPI and metabolize it into SCFAs. Moreover, after 48 h of KFPI fecal fermentation, it was observed that KFPI contributed to maintaining the balance of gut microbiota by promoting the proliferation of beneficial bacteria like Bacteroides, Lactobacillus, and Bifidobacterium, while inhibiting the unfavorable bacteria like Bilophila. In summary, this study offers a comprehensive exploration of in vitro digestion and fecal fermentation characteristics of KFP, providing valuable insights for potential development of KFP as a prebiotic for promoting intestinal health.
猕猴桃渣富含具有多种生物活性和益生元潜力的多糖。本研究通过体外模拟唾液-胃肠消化和粪便发酵深入研究了猕猴桃渣多糖（KFP）的消化行为和发酵特性。结果表明，KFP 经模拟消化后，其分子量降低了 4.7%，还原糖（C）增加了 9.5%。然而，单糖组成和傅里叶变换红外光谱特征没有明显变化，表明 KFP 未被消化。此外，即使经过唾液-胃肠消化，KFP 仍保留体外降血脂和降血糖活性。随后，粪便发酵显著改变了不可消化的 KFP（KFPI）的理化性质，特别是 C 减少了 89.71%。这表明肠道微生物群可以分解 KFPI 并将其代谢成 SCFAs。此外，在 KFPI 粪便发酵 48 小时后，观察到 KFPI 通过促进有益菌如拟杆菌、乳杆菌和双歧杆菌的增殖，同时抑制不利菌如Bilophila，有助于维持肠道微生物群的平衡。总之，本研究全面探讨了 KFP 的体外消化和粪便发酵特性，为将 KFP 开发为促进肠道健康的益生元提供了有价值的见解。
