Laboratory of Proteins and Microorganisms Applied to Chemistry, Institute of Chemistry, UFRGS, Av. Bento Gonçalves 9500, CEP 91501-970, Porto Alegre, RS, Brazil.
Institute of Chemistry, UFRGS, Av. Bento Gonçalves 9500, CEP 91501-970 Porto Alegre, RS, Brazil.
Int J Biol Macromol. 2023 Dec 31;253(Pt 6):127134. doi: 10.1016/j.ijbiomac.2023.127134. Epub 2023 Sep 28.
Oral mucosal ulcerations expose connective tissue to different pathogens and this can progress to systemic infection. This study aimed to synthesize environmentally-friendly films with chitosan and protic ionic liquids, possessing mucoadhesive properties, activity against opportunistic microorganisms, enhanced malleability and mechanical resistance to be used as a wound dressing on the oral mucosa. Therefore, films with chitosan and 10, 35, and 50 % (wt/wt) of 2-hydroxy diethylammonium lactate, salicylate, and maleate protic ionic liquids were synthesized. Thickness measurements and mechanical properties analysis were performed. In addition, oral mucoadhesion, antimicrobial activity, and cytotoxicity properties were investigated. Results showed that the addition of 35wt% and 50wt% of all kinds of protic ionic liquids tested presented significant improvements in film thickness and mechanical properties. Films based on chitosan and the protic ionic liquid 2-hydroxy diethylammonium salicylate at percentages of 35 and 50wt% exhibited superior mucoadhesive properties, antimicrobial activity on opportunistic microorganisms and an improvement in their flexibility after immersion in synthetic saliva. Cytotoxicity results suggest that all kinds of chitosan/protic ionic liquids films tested are safe for intra-oral use. Therefore, the results of this study indicate that these materials could be good candidates for efficient and environmentally-friendly wound dressing films on the oral mucosa.
口腔黏膜溃疡使结缔组织暴露于不同的病原体，这可能会导致全身感染。本研究旨在合成具有壳聚糖和质子离子液体的环保型薄膜，具有粘膜粘附性能、抗机会性微生物活性、增强的柔韧性和机械强度，可作为口腔黏膜的伤口敷料。因此，合成了壳聚糖和 10%、35%和 50%（wt/wt）的 2-羟二乙氨基乳酸盐、水杨酸盐和马来酸盐质子离子液体的薄膜。进行了厚度测量和机械性能分析。此外，还研究了口腔粘膜粘附性、抗菌活性和细胞毒性。结果表明，添加 35wt%和 50wt%的各种质子离子液体都显著提高了薄膜的厚度和机械性能。基于壳聚糖和质子离子液体 2-羟二乙氨基水杨酸盐的薄膜在 35wt%和 50wt%的比例下表现出更好的粘膜粘附性能、对机会性微生物的抗菌活性以及在浸入合成唾液后的柔韧性改善。细胞毒性结果表明，所有测试的壳聚糖/质子离子液体薄膜对口腔内使用都是安全的。因此，本研究结果表明，这些材料可能是口腔粘膜高效环保伤口敷料薄膜的良好候选材料。
