DJK-5 和洗必泰对口腔生物膜胞外多糖体积和 pH 的影响。

Effects of DJK-5 and chlorhexidine on exopolysaccharide volume and pH in oral biofilms.

机构信息

Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei Province, China.

Department of Oral Biological & Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, Canada.

出版信息

BMC Oral Health. 2023 Sep 30;23(1):705. doi: 10.1186/s12903-023-03381-5.

DOI:10.1186/s12903-023-03381-5

PMID:37777729

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10544135/

Abstract

BACKGROUND

Exopolysaccharides (EPS) are essential constituents of the extracellular matrix within oral biofilms and are significantly influenced by the local microenvironment. This study aimed to investigate the impact of two distinct antimicrobial agents, DJK-5 and chlorhexidine (CHX), on the EPS volume and pH levels in oral biofilms.

METHODS

Oral biofilms obtained from two donors were cultured on hydroxyapatite discs for durations of 3 days, 1 week, 2 weeks, 3 weeks, and 4 weeks. Subsequently, these biofilms were subjected to treatment with 10 µg/mL DJK-5 or 2% CHX for 3 min. The impact of these antimicrobial treatments on factors such as the proportion of dead bacterial, in situ pH, and EPS volume within the biofilms was assessed using corresponding fluorescent probes. The examination was carried out utilizing confocal laser scanning microscopy, and the resulting images were analyzed with a focus on the upper and lower layers of the biofilm, respectively.

RESULTS

DJK-5 exhibited a more potent bactericidal effect compared to CHX across the 3-day to 4-week duration of the biofilm (P < 0.05). The biofilms were acidic, with the upper layer being less acidic than the lower layer (P < 0.05). Both antimicrobial agents increased the pH, but DJK-5 had a greater effect than CHX (P < 0.05). The volume of EPS was significantly lower in DJK-5 treated biofilms compared to that of CHX, regardless of age or layer (P < 0.05).

CONCLUSION

DJK-5 exhibited superior effectiveness in reducing viable bacteria and EPS volume, as well as in raising extracellular pH, as compared to chlorhexidine.

摘要

背景

胞外多糖（EPS）是口腔生物膜细胞外基质的重要组成部分，其含量受到局部微环境的显著影响。本研究旨在探讨两种不同的抗菌剂 DJK-5 和洗必泰（CHX）对口腔生物膜 EPS 体积和 pH 值的影响。

方法

从两名供体中获取口腔生物膜，在羟基磷灰石片上培养 3 天、1 周、2 周、3 周和 4 周。然后，用 10 μg/mL 的 DJK-5 或 2% CHX 处理这些生物膜 3 分钟。使用相应的荧光探针评估这些抗菌处理对生物膜内死细菌比例、原位 pH 和 EPS 体积等因素的影响。利用共聚焦激光扫描显微镜进行检查，分别对生物膜的上下层进行分析。

结果

与 CHX 相比，DJK-5 在 3 天至 4 周的生物膜培养期间表现出更强的杀菌效果（P<0.05）。生物膜呈酸性，上层比下层酸度稍低（P<0.05）。两种抗菌剂均能提高 pH 值，但 DJK-5 的效果优于 CHX（P<0.05）。与 CHX 相比，DJK-5 处理的生物膜中 EPS 体积明显降低，与生物膜的年龄或层无关（P<0.05）。