Key Laboratory of Oral Biomedical Research of Zhejiang Province, School of Stomatology, Stomatology Hospital, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Cancer Center of Zhejiang University, Hangzhou 310000, China; Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver V6T 1Z3, Canada.
Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver V6T 1Z3, Canada; The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
J Dent. 2023 Jul;134:104552. doi: 10.1016/j.jdent.2023.104552. Epub 2023 May 16.
To evaluate the dynamics of killing of oral multispecies biofilms grown on dental restorative materials by commercially available mouthrinses and a d-enantiomeric peptide.
Four composite resins (3 M Supreme, 3 M Supreme flow, Kerr Sonicfill, and Shofu Beautifil II) and one glass ionomer (GC Fuji II) were used as restorative materials. Plaque biofilms were grown on the surfaces of restorative material discs for 1 week. The surface roughness and biofilm attachment were assessed by atomic force microscopy and scanning electron microscopy. One-week-old biofilms grown anaerobically at 37 °C were exposed to each of five solutions for one minute (twice daily for seven days): Listerine Total care and Paroex Gum mouthrinses, 0.12% chlorhexidine, 0.001% d-enantiomeric peptide DJK-5, and sterile water. The dynamic variation of the biovolume of the biofilms and the percentage of dead bacteria were monitored and analyzed using confocal laser scanning microscopy.
All restorative materials had similar surface roughness with intact biofilm attachment. The percentage of dead bacteria and biovolume of biofilms treated by each oral rinse solution remained constant between days 1 and 7, with no statistically significant difference. DJK-5 showed the highest percentage of dead bacteria (up to 75.7%; cf. ∼20-40% for other mouthrinses) of all solutions tested within 7 days.
DJK-5 outperformed conventional mouthrinses in killing bacteria in oral multispecies biofilms grown on dental restorative materials.
The antimicrobial peptide DJK-5 is effective against oral biofilms and serves as a promising candidate for the development of future mouthrinses to improve long-term oral hygiene.
评估市售漱口水和 D-对映体肽对牙修复材料上生长的口腔多菌种生物膜的杀菌动力学。
选用 4 种复合树脂（3M Supreme、3M Supreme flow、Kerr Sonicfill 和 Shofu Beautifil II）和 1 种玻璃离子体（GC Fuji II）作为修复材料。将菌斑生物膜在修复材料圆盘表面培养 1 周。采用原子力显微镜和扫描电子显微镜评估表面粗糙度和生物膜附着。将在 37°C 下厌氧培养 1 周的生物膜暴露于 5 种溶液中 1 分钟（每天两次，持续 7 天）：Listerine Total care 和 Paroex Gum 漱口水、0.12%洗必泰、0.001% D-对映体肽 DJK-5 和无菌水。使用共聚焦激光扫描显微镜监测和分析生物膜的生物体积和死亡细菌百分比的动态变化。
所有修复材料均具有相似的表面粗糙度和完整的生物膜附着。用每种漱口液处理的细菌死亡百分比和生物膜生物体积在第 1 天至第 7 天之间保持不变，无统计学差异。在 7 天内，所有测试溶液中，DJK-5 显示出最高的死亡细菌百分比（高达 75.7%；相比之下，其他漱口水约为 20-40%）。
DJK-5 在杀死牙修复材料上生长的口腔多菌种生物膜中的细菌方面优于常规漱口水。
抗菌肽 DJK-5 对口腔生物膜有效，是开发未来漱口水以改善长期口腔卫生的有前途的候选药物。
