Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai, Japan.
MAC Gifu Research Institute, MicroAlgae Corporation , Gifu, Japan.
Biosci Biotechnol Biochem. 2023 Dec 19;88(1):16-25. doi: 10.1093/bbb/zbad138.
We previously demonstrated that dietary supplementation with Dunaliella tertiolecta (DT) increases uncoupling protein 1 (UCP1) expression in brown adipose tissue (BAT) and improves diet-induced obesity (DIO) in C57BL/6 J mice at thermoneutrality (30 °C). Here, we investigated whether DT improves DIO in a thermoneutral UCP1-deficient (KO) animal. KO mice were fed a high-fat diet supplemented with DT for 12 weeks. Compared to control group without DT, body weight was significantly reduced in DT group with no difference in food intake. Dunaliella tertiolecta-supplemented mice exhibited lower adiposity and well-maintained multilocular morphology in BAT, in which a significant increase in gene expression of PR domain containing 16 was detected in DT group compared to control group. Moreover, increase in UCP2 level and/or decrease in ribosomal protein S6 phosphorylation were detected in adipose tissues of DT group relative to control group. These results suggest that DT supplementation improves DIO by stimulating UCP1-independent energy dissipation at thermoneutrality.
我们之前的研究表明，在 30°C 等热中性条件下，小球藻（Dunaliella tertiolecta，DT）补充剂可增加棕色脂肪组织（BAT）中的解偶联蛋白 1（UCP1）表达，并改善 C57BL/6J 小鼠的饮食诱导肥胖（DIO）。在此，我们研究了 DT 是否能改善 UCP1 缺陷（KO）动物的 DIO。KO 小鼠喂食高脂肪饮食并补充 DT 12 周。与未添加 DT 的对照组相比，DT 组的体重显著降低，而食物摄入量无差异。添加 DT 的小球藻补充剂表现出较低的肥胖症和维持良好的多房形态，在 BAT 中检测到 PR 域包含 16 的基因表达显著增加，与对照组相比。此外，与对照组相比，DT 组的脂肪组织中 UCP2 水平升高和/或核糖体蛋白 S6 磷酸化减少。这些结果表明，DT 补充剂通过在等热中性条件下刺激非 UCP1 依赖性能量耗散来改善 DIO。
