Department of Dentistry, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
Department of Genetics, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia; Center for Harm Reduction of Biological and Chemical Hazards, Faculty of Medical Sciences University of Kragujevac, Serbia.
Arch Oral Biol. 2023 Dec;156:105808. doi: 10.1016/j.archoralbio.2023.105808. Epub 2023 Sep 20.
To investigate the influence of strain differences in immune response on the pathogenesis of oral candidiasis in Dark Agouti (DA) and Albino Oxford (AO) inbred strains of rats.
Seventy male 8-weeks old DA and AO rats were inoculated with Candida albicans to induce three different experimental models of oral candidiasis, one immunocompetent and two immunocompromised models. The animals were sacrificed after 16 days from the beginning of the experiment followed by collecting the samples of the tongue dorsum and blood for histopathological (PAS and H&E staining), immunohistochemical, qRT-PCR, and oxidative stress analyses.
Histopathological and immunohistochemical analyses revealed lower levels of epithelial colonization, epithelial damage, and inflammatory infiltration in DA compared to AO strain of rats. DA rats had fewer CD45, CD68, and CD3 positive cells but more HIS 48 positive cells than AO rats. The expressions of IL-1β, TNFα, IFN-γ, IL-10 and TGF-β1 were consistently higher in DA strain across all experimental models. However, the expressions of IL-4 and IL-17 differed inconsistently between DA and AO strain in various experimental models. Strain differences were observed in levels of prooxidative hydrogen peroxide and lipid peroxidation, with higher levels presented in AO rats compared to DA rats, while antioxidative parameters presented little yet inconsistent difference between strains.
DA strain of rats consistently presented lower susceptibility to oral infection with C. albicans compared to AO strain with robust Th1/Th17 immune response indicating the importance of the genetic background on the development of oral candidiasis.
研究免疫反应的品系差异对 Dark Agouti（DA）和 Albino Oxford（AO）近交系大鼠口腔念珠菌病发病机制的影响。
将 70 只 8 周龄雄性 DA 和 AO 大鼠接种白念珠菌，诱导三种不同的口腔念珠菌病实验模型，一种免疫功能正常和两种免疫功能低下的模型。实验开始后 16 天处死动物，采集舌背和血液样本进行组织病理学（PAS 和 H&E 染色）、免疫组织化学、qRT-PCR 和氧化应激分析。
组织病理学和免疫组织化学分析显示，DA 大鼠的上皮定植、上皮损伤和炎症浸润水平低于 AO 大鼠。与 AO 大鼠相比，DA 大鼠的 CD45、CD68 和 CD3 阳性细胞较少，但 HIS 48 阳性细胞较多。在所有实验模型中，DA 大鼠的 IL-1β、TNFα、IFN-γ、IL-10 和 TGF-β1 的表达始终较高。然而，在不同的实验模型中，DA 和 AO 大鼠之间的 IL-4 和 IL-17 表达存在不一致的差异。在过氧化氢和脂质过氧化等促氧化水平上观察到品系差异，AO 大鼠的水平高于 DA 大鼠，而抗氧化参数在品系之间差异不大。
与 AO 大鼠相比，DA 大鼠对白念珠菌口腔感染的易感性始终较低，具有更强的 Th1/Th17 免疫反应，表明遗传背景对口腔念珠菌病的发展很重要。
