Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education / College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
Pest Manag Sci. 2024 Feb;80(2):744-755. doi: 10.1002/ps.7806. Epub 2023 Oct 11.
Mythimna loreyi is an important agricultural pest with a sensitive sex pheromone communication system. To clarify the pheromone binding proteins (PBPs) and pheromone receptors (PRs) involved in sex pheromone perception is important for both understanding the molecular olfactory mechanism and developing a new pest control strategy in M. loreyi.
First, the electroantennogram (EAG) assay showed that male M. loreyi displayed the highest response to the major sex pheromone component Z9-14:Ac, and higher responses to two minor components, Z7-12:Ac and Z11-16:Ac. Second, the fluorescence competition binding assay showed that PBP1 bound all three pheromones and other tested compounds with high or moderate affinity, while PBP2 and PBP3 each bound only one pheromone component and few other compounds. Third, functional study using the Xenopus oocyte system demonstrated that, of the six candidate PRs, PR2 was weakly sensitive to the major pheromone Z9-14:Ac, but was strongly sensitive to pheromone analog Z9-14:OH; PR3 was strongly and specifically sensitive to a minor component Z7-12:Ac; PR4 and OR33 were both weakly sensitive to another minor component, Z11-16:Ac. Finally, phylogenetic relationship and ligand profiles of PRs were compared among six species from two closely related genera Mythimna and Spodoptera, suggesting functional shifts of M. loreyi PRs toward Spodoptera PRs.
Functional differentiations were revealed among three PBPs and six PRs in sex pheromone perception, laying an important basis for understanding the molecular mechanism of sex pheromone perception and for developing new control strategies in M. loreyi. © 2023 Society of Chemical Industry.
稻纵卷叶螟是一种重要的农业害虫，其性信息素通讯系统十分敏感。阐明参与性信息素感知的信息素结合蛋白（PBPs）和信息素受体（PRs）对于理解分子嗅觉机制和开发稻纵卷叶螟新的害虫防治策略都很重要。
首先，触角电位（EAG）测定表明，雄虫稻纵卷叶螟对主要性信息素成分 Z9-14：Ac 表现出最高的反应，对两个次要成分 Z7-12：Ac 和 Z11-16：Ac 也有较高的反应。其次，荧光竞争结合测定表明，PBP1 与所有三种信息素和其他测试化合物具有高或中等亲和力，而 PBP2 和 PBP3 各自仅与一种信息素成分和少数其他化合物结合。第三，使用非洲爪蟾卵母细胞系统进行的功能研究表明，在六个候选 PR 中，PR2 对主要信息素 Z9-14：Ac 表现出较弱的敏感性，但对信息素类似物 Z9-14：OH 表现出强烈的敏感性；PR3 对一个次要成分 Z7-12：Ac 表现出强烈且特异性的敏感性；PR4 和 OR33 对另一个次要成分 Z11-16：Ac 都表现出较弱的敏感性。最后，对来自两个密切相关的属稻纵卷叶螟和夜蛾属的 6 种昆虫的 PRs 的系统发育关系和配体谱进行了比较，表明稻纵卷叶螟 PRs 向夜蛾属 PRs 的功能转变。
在性信息素感知中，三个 PBP 和六个 PR 之间显示出功能分化，为理解性信息素感知的分子机制和开发稻纵卷叶螟新的防治策略奠定了重要基础。© 2023 化学工业协会。
