The Ross Laboratory for Studies in Neural Birth Defects, Department of Medical Neurobiology, Institute for Medical Research - Israel-Canada, The Hebrew University-Hadassah Medical School, P.O. Box 12272, 91120, Jerusalem, Israel.
Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Sci Rep. 2023 Oct 1;13(1):16499. doi: 10.1038/s41598-023-43593-x.
Although the effects of paternal exposure to insults on the offspring received limited attention in the past, it is currently gaining interest especially after understanding the mechanisms which may mediate such exposure effects. In the current study, the well-controlled avian model (Fayoumi) was utilized to investigate the effects of paternal exposure to the developmental insult, chlorpyrifos on the offspring's gene expression via mRNA and small RNA sequencing. Numerous mRNA gene expression changes were detected in the offspring after paternal exposure to the developmental insult, especially in genes related to neurogenesis, learning and memory. qPCR analysis of several genes, that were significantly changed in mRNA sequencing, confirmed the results obtained in mRNA sequencing. On the other hand, small RNA sequencing did not identify significant microRNA genes expression changes in the offspring after paternal exposure to the developmental insult. The effects of the paternal exposure were more pronounced in the female offspring compared to the male offspring. The results identified expression alterations in major genes (some of which were pertinent to the functional changes observed in other forms of early developmental exposure) after paternal insult exposure and provided a direction for future studies involving the most affected genes.
虽然过去父亲暴露于侮辱对后代的影响受到的关注有限，但目前人们对此越来越感兴趣，特别是在了解了可能介导这种暴露效应的机制之后。在本研究中，利用经过良好控制的禽类模型（Fayoumi），通过 mRNA 和小 RNA 测序，研究了父亲暴露于发育性侮辱（毒死蜱）对后代基因表达的影响。父亲暴露于发育性侮辱后，后代的许多 mRNA 基因表达发生变化，特别是与神经发生、学习和记忆相关的基因。qPCR 分析了几个在 mRNA 测序中显著变化的基因，证实了 mRNA 测序中获得的结果。另一方面，小 RNA 测序未发现父亲暴露于发育性侮辱后后代中显著的 microRNA 基因表达变化。与雄性后代相比，父亲暴露对雌性后代的影响更为明显。这些结果确定了父亲受辱后主要基因的表达变化（其中一些与其他形式的早期发育暴露观察到的功能变化有关），为未来涉及受影响最大的基因的研究提供了方向。
