改进的错配扩增突变检测-PCR 用于区分猪链球菌 2 型和 1/2 型。
Improvement of the mismatch amplification mutation assay-PCR for discrimination between Streptococcus suis serotypes 2 and 1/2.
机构信息
Miyazaki Prefectural Livestock Hygiene Service Center, 3151-1 Shimonaka, Sadoharacho, Miyazaki 880-0212, Japan.
Fukuoka Prefectural Chuo Livestock Hygiene Service Center, 4-14-5 hakozakifutou, Higashi-ku, Fukuoka 812-0051, Japan.
出版信息
J Microbiol Methods. 2023 Nov;214:106828. doi: 10.1016/j.mimet.2023.106828. Epub 2023 Sep 30.
A mismatch amplification mutation assay (MAMA)-PCR, which detects a single-nucleotide polymorphism contributed to serological difference between Streptococcus suis serotypes 2 and 1/2, is used to discriminate between these serotypes. The present study reports unusual serotype 1/2 isolates untypable by the MAMA-PCR and improvement of the MAMA-PCR for typing such isolates.
一种错配扩增突变检测（MAMA）-PCR 被用于区分猪链球菌血清型 2 型和 1/2 型，该方法检测到一个导致血清型 2 型和 1/2 型之间血清学差异的单核苷酸多态性。本研究报告了一些不典型的 1/2 型分离株，这些分离株无法通过 MAMA-PCR 进行分型，并改进了 MAMA-PCR 用于对这些分离株进行分型。
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