Mine T, Kojima I, Kimura S, Ogata E
Biochem Biophys Res Commun. 1986 Oct 15;140(1):107-13. doi: 10.1016/0006-291x(86)91064-8.
Effects of phenylephrine, vasopressin and angiotensin II on cytoplasmic free calcium concentration, [Ca2+]c, were examined by monitoring aequorin bioluminescence in isolated hepatocytes preloaded with aequorin. In the presence of 0.5 mM calcium in the medium, the pattern of changes in aequorin bioluminescence induced by phenylephrine was different from that induced by vasopressin or angiotensin II. When extracellular calcium concentration was reduced to 1 microM, however, these three agents induced identical changes in aequorin bioluminescence. These results suggest that the mode of action of phenylephrine on cytoplasmic free calcium concentration differs from that of either vasopressin or angiotensin II and that the difference in ability to increase calcium influx may account for the distinct patterns induced by these agents.
通过监测预先装载水母发光蛋白的离体肝细胞中水母发光蛋白的生物发光，研究了去氧肾上腺素、血管加压素和血管紧张素II对细胞质游离钙浓度[Ca2+]c的影响。在培养基中存在0.5 mM钙的情况下，去氧肾上腺素诱导的水母发光蛋白生物发光变化模式与血管加压素或血管紧张素II诱导的不同。然而，当细胞外钙浓度降至1 microM时，这三种试剂诱导的水母发光蛋白生物发光变化相同。这些结果表明，去氧肾上腺素对细胞质游离钙浓度的作用模式不同于血管加压素或血管紧张素II，并且增加钙内流能力的差异可能解释了这些试剂诱导的不同模式。
