胚胎鸡肝脏δ-氨基-γ-酮戊酸合酶产生的调节：睾酮和血红素对该酶mRNA的影响。

Regulation of production of embryonic chick liver delta-aminolevulinate synthase: effects of testosterone and of hemin on the mRNA of the enzyme.

作者信息

Drew P D, Ades I Z

出版信息

Biochem Biophys Res Commun. 1986 Oct 15;140(1):81-7. doi: 10.1016/0006-291x(86)91060-0.

Abstract

The effects of testosterone and of hemin on the concentration of the mRNA of embryonic chick liver ALA synthase were investigated. Using cDNA-RNA liquid hybridization analyses, we determined that testosterone, when injected into the fluid surrounding chick embryos, caused a dose-dependent increase in the concentration of ALA synthase mRNA in liver. Similarly, addition of testosterone (5 micrograms/ml) or of 75 micrograms/ml of allylisopropylacetamide (AIA) into the medium of chick embryo hepatocytes maintained in culture caused an increase in the concentration of ALA synthase mRNA. Hemin (2 or 5 microM), when added to the culture medium, inhibited the elevations of ALA synthase mRNA concentration brought on by testosterone and by AIA.

摘要

研究了睾酮和血红素对胚胎鸡肝脏δ-氨基-γ-酮戊酸合酶（ALA合酶）mRNA浓度的影响。通过cDNA-RNA液相杂交分析，我们确定，当将睾酮注射到鸡胚周围的液体中时，会导致肝脏中ALA合酶mRNA浓度呈剂量依赖性增加。同样，向培养的鸡胚肝细胞培养基中添加睾酮（5微克/毫升）或75微克/毫升的烯丙基异丙基乙酰胺（AIA）会导致ALA合酶mRNA浓度增加。当向培养基中添加血红素（2或5微摩尔）时，可抑制睾酮和AIA引起的ALA合酶mRNA浓度升高。